应用aurintricarboxylic酸以提高CHO细胞的性能
Chao Tao1, Jizhou Meng1, Dan Wen1
1Cell Culture Process Development (CCPD), WuXi Biologics, 31 Yiwei Road, Waigaoqiao Free Trade Zone, Shanghai, 200131, China.
Biotechnology letters
|July 22, 2025
概括
奥林特里碳酸 (ATA) 在生物制药制造中增强了中国仓鼠卵巢 (CHO) 细胞活力和乳酸性能. 使用ATA的新型种子列车过程也提高了细胞性能和生产力.
科学领域:
- 生物技术是生物技术.
- 细胞培养 细胞培养
- 生物制药制造业 生物制药制造业
背景情况:
- 中国仓鼠卵巢 (CHO) 细胞是生产蛋白质治疗药物的主要宿主.
- 细胞问题,如活力下降和有毒代谢物积累,阻碍了生产力.
- 奥林特里碳酸 (ATA) 因其抗氧化和促进细胞生长的特性而受到研究.
研究的目的:
- 评估ATA作为一种增剂,以提高CHO细胞的性能和蛋白质生产.
- 研究ATA在减轻细胞应激和增强活力的作用机制.
- 开发一种新的ATA应用策略,以改善细胞培养过程.
主要方法:
- 用不同的ATA度和添加策略培养两个CHO细胞克隆的料批培养.
- 评估细胞活力,乳酸积累和活性氧物种 (ROS) 水平.
- 同焦显微镜可视化细胞内ATA局部化和ROS抑制.
主要成果:
- ATA在两种CHO细胞克隆中显著增加了至少10%的收获活力.
- 在晚期食批量培养期间,通过ATA添加抑制了乳酸积累.
- 新的ATA集成到种子列车过程中提高了种子性能和整体生产力.
- 观察到ATA进入CHO细胞并减少细胞内ROS生成.
结论:
- ATA有效地提高了CHO细胞的性能,维持了活力并改善了代谢概况.
- 使用ATA的新型超级种子列车工艺为生物制药制造提供了显著的好处.
- 这项研究促进了对ATA在优化基于CHO细胞的生物生产中的作用的理解.
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