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萨尔科西斯蒂斯物种:水牛 (Bubalus bubalis) 中的分子鉴定和血清流行

Nagwa I Toaleb¹, Raafat M Shaapan², Haitham Elaadli³

¹Parasitology and Animal Diseases Department, Veterinary Research Institute, National Research Centre, El Buhouth St., Dokki, Giza, Egypt.

BMC veterinary research

|July 22, 2025

概括

萨尔科西斯蒂斯 spp. 的一种. 用分子和组织学方法确定了埃及水牛的感染. 一种已开发的ELISA测试显示,在活体动物中检测囊炎抗体的准确性很高.

科学领域:

兽医寄生虫学兽医寄生虫学
通过食物传播的动物感染病.
分子生物学分子生物学

背景情况:

萨尔科西斯蒂斯 spp. 的一种. 它们是感染肉类动物的动物性虫原生动物,造成公共卫生风险和经济损失.
水牛 (Bubalus bubalis) 是Sarcocystis的重要牲畜储备,特别是在埃及.

研究的目的:

进行Sarcocystis spp.的分子鉴定,组织学检查和血清流行率评估. 在埃及水牛中. 在埃及水牛中. 在埃及水牛中.
为了评估间接ELISA测试对萨尔科囊抗体检测的有效性.

主要方法:

在屠宰的水牛中对Sarcocystis巨囊的宏观和组织学检查.
使用PCR放大和测序18S rRNA基因的分子鉴定.
使用S. fusiformis全囊抗原的间接ELISA血清分析.

主要成果:

观察到的宏观感染率为27.3%,在老雌雄牛和食道中患病率更高.
三种Sarcocystis物种 (S.buffalonis,S.fusiformis,S.hirsuta-like) 在分子上被确定.
间接ELISA检测显示,对检测囊炎抗体具有高灵敏度 (97.6%) 和特异性 (100%).

结论:

这项研究提供了关于Sarcocystis spp.的全面数据. 在埃及水牛中,包括分子和组织学发现.
使用S. fusiformis抗原的间接ELISA是一种可靠的工具,用于在活体动物中对Sarcocystis感染进行血清学诊断.

关键词:

囊瘤是一种囊瘤.雄牛的牛,就是水牛.艾丽莎·伊丽莎·伊丽莎分子分析分子分析这是一个SDS-PAGE页面.西方黑斑是因为西方黑斑.

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