相关实验视频

Updated: Sep 14, 2025

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana

Published on: May 21, 2019

挥发性2-乙醇和β-环酸触发了与防御相关的转录和代谢变化,该变化发生在葡萄藤树叶中,对抗菌

Sara Avesani^1,2,3, Valentina Lazazzara³, Matteo Buti⁴

¹Center for Agriculture Food Environment (C3A), University of Trento, San Michele all'Adige, Italy.

Physiologia plantarum

|July 23, 2025

概括

挥发性有机化合物 (VOC),如2-乙醇和β-环醇,可以诱导葡萄树对菌的抵抗力. 这些VOC通过改变基因表达和代谢途径来重新编程植物防御,为疾病管理提供了一种新的策略.

科学领域:

植物病理学植物病理学
植物生物化学植物生物化学
分子生物学分子生物学

背景情况:

葡萄树叶在感染病原体时产生挥发性有机化合物 (VOC).
2-乙醇和β-cyclocitral是由Plasmopara viticola触发的VOC,但它们在植物耐药性中的作用尚不清楚.

研究的目的:

为了研究由2-乙醇和β-环酸治疗诱导的转录和代谢变化.
为了澄清这些VOC如何激活植物对Plasmopara viticola的抗药性.

主要方法:

葡萄树叶盘用2-乙醇和β-环醇进行处理,并用Plasmopara viticola进行注射.
使用RNA-Seq进行的转录组分析是在接种疫苗后1天和6天进行的.
进行了代谢组概况和转录组和代谢组数据之间的相关性分析.

主要成果:

两种VOC治疗都显著降低了菌的严重程度.
RNA-Seq揭示了防御,氧化应激,二次新陈代谢,信号转导和转录相关基因的调节.
代谢分析显示了氨基酸,碳水化合物,黄类,类和类代谢的重编程,与与防御有关的特定化合物有关.

结论:

2-乙醇和β-环醇作为葡萄树中抗菌的耐药性诱导剂.
这些VOC会触发复杂的转录和代谢重编程,增强植物的防御机制.

关键词:

葡萄酒 (Vitis vinifera) 是一种葡萄酒.诱导的阻力诱导的阻力转录组分析转录组分析没有针对性的代谢学分析分析.挥发性有机化合物挥发性有机化合物

更多相关视频

Detection of Histone Modifications in Plant Leaves

Detection of Histone Modifications in Plant Leaves

Published on: September 23, 2011

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

The Terroir Concept Interpreted through Grape Berry Metabolomics and Transcriptomics

Published on: October 5, 2016

相关概念视频

Adaptations that Reduce Water Loss

Adaptations that Reduce Water Loss

Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.

Introduction to Plant Diversity

Introduction to Plant Diversity

From Water to Land

Regulation of Transpiration by Stomata

Regulation of Transpiration by Stomata

During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Cycloaddition Reactions: MO Requirements for Photochemical Activation

Cycloaddition Reactions: MO Requirements for Photochemical Activation

Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.

Defenses Against Pathogens and Herbivores

Defenses Against Pathogens and Herbivores

Plants present a rich source of nutrients for many organisms, making it a target for herbivores and infectious agents. Plants, though lacking a proper immune system, have developed an array of constitutive and inducible defenses to fend off these attacks.