相关实验视频

Updated: Sep 14, 2025

Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation

Potentiation of Anticancer Antibody Efficacy by Antineoplastic Drugs: Detection of Antibody-drug Synergism Using the Combination Index Equation

Published on: January 19, 2019

非转化E7蛋白与TLR7激动剂结合的抗瘤效应

Maryam Mashhadi Abolghasem Shirazi¹, Seyed Mehdi Sadat², Nasser Hashemi Goradel³

¹Department of Molecular Virology, Pasteur Institute of Iran, Tehran, Iran AND Department of Microbiology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. mersedeh_shirazi@yahoo.com.

Iranian journal of allergy, asthma, and immunology

|July 23, 2025

概括

一种新型的人类乳头瘤病毒 (HPV) E7突变蛋白 (E7GGG) 与阿尔达拉辅助剂相结合,显示出作为治疗疫苗的前景. 在小鼠模型中,这种组合有效诱导免疫反应并抑制瘤生长.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
疫苗学疫苗学疫苗学

背景情况:

人类乳头瘤病毒 (HPV) 诱导的宫癌治疗疫苗在引起强大和持续的免疫反应方面面临挑战.
E7蛋白是HPV疫苗的关键目标,但其转变性质可能会使疫苗开发复杂化.

研究的目的:

为了评估修改后的HPV16 E7蛋白 (E7GGG) 的疫苗潜力,消除了转化性质,但保留了抗原性,与阿尔达拉 (局部伊米基莫德) 辅助剂结合.
评估HPV16-E7GGG和Aldara在TC-1小鼠模型中诱导幽默和细胞免疫反应并抑制瘤生长的能力.

主要方法:

再组合的HPV16-E7GGG和野生型E7蛋白被表达和净化.
蛋白质与阿尔达拉辅助剂结合并给C57BL小鼠.
测量了免疫反应,包括IgG,IL-4,IL-17,IFN-γ和CTL活性. 在TC-1小鼠模型中监测瘤生长.

主要成果:

与阿尔达拉辅助剂结合的HPV16-E7GGG抗原成功诱导了显著的幽默和细胞免疫反应.
免疫反应的特征是T助手1 (TH1) 介导的通路,由IL-17和IFN-γ水平的升高表明.
与对照组相比,免疫组观察到显著抑制瘤生长.

结论:

再组合的HPV16-E7GGG蛋白与阿尔达拉辅助剂结合,显示出作为治疗性疫苗候选人对抗HPV诱导的癌症的潜力.
疫苗策略引发了TH1偏倚的免疫反应,这对于有效的抗瘤免疫是至关重要的.
需要进一步的研究,以探索其在宫癌治疗中的临床适用性.

关键词:

剧情片 (Epitopes) 是一种表情片.人类乳头瘤病毒16 艾米基基莫德 (Imiquimod) 是一种药物.子宫瘤是什么子宫瘤疫苗疫苗是一种疫苗.

更多相关视频

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Novel Protocol for Generating Physiologic Immunogenic Dendritic Cells

Published on: May 17, 2019

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Preparation, Characteristics, Toxicity, and Efficacy Evaluation of the Nasal Self-Assembled Nanoemulsion Tumor Vaccine In Vitro and In Vivo

Published on: September 28, 2022

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...