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相关概念视频

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

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Window on a Microworld: Simple Microfluidic Systems for Studying Microbial Transport in Porous Media
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介质辅助翻转控制了细菌在复杂的流体中进行探索.

Héctor Urra1, Thierry Darnige1, Xavier Benoit-Gonin1

  • 1Laboratoire PMMH-ESPCI Paris, PSL Research University, Sorbonne Université and Université Paris cité, 7, quai Saint-Bernard, Paris, France. hectorignaciou@gmail.com.

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概括

在非牛顿流体中的细菌探索是通过介质辅助进行的,而不仅仅是生物驱动的. 当地的机械特性取代了大肠杆菌.

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科学领域:

  • 微生物学 微生物学
  • 类风病学 类风病学 类风病学
  • 生物物理学的生物物理.

背景情况:

  • 许多含有细菌的自然液体都表现出非牛顿式的风湿学.
  • 了解这种复杂的介质中的细菌空间探索至关重要.

研究的目的:

  • 为了研究大肠杆菌的移动性是如何受到可调节的宏观rheology的影响.
  • 在牛顿式与产量压力流体中描述细菌导航.

主要方法:

  • 设计了一种可调节的风湿学移动性介质,使用碳质颗粒.
  • 采用3D拉格朗追踪来分析个体细菌轨迹.
  • 野生型大肠杆菌与一个平滑的跑者突变菌进行了比较.

主要成果:

  • 观察到游泳速度,持久性和扩散性的变化,随着碳合物度的增加.
  • 在较高度下发现了一种运动障碍.
  • 证明了当地的机械障碍和阻力会覆盖运行和的导航.

结论:

  • 在非牛顿流体中的细菌探索受到介质性质的显著影响.
  • 一个"中等辅助"的探索场景与停止-and-go动力学是特征.
  • 旗束的灵活性与这种介质辅助运动有关.