通过将生物信息学与机器学习分析结合起来,识别出与败血症相关的ARDS中的诊断生物标志物和治疗点

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Tingting Liu¹, Ling Gao¹, Xiaoyan Li²

¹Department of Respiratory and Critical Care Medicine, Third Hospital of Shanxi Medical University, Shanxi Bethune Hospital, Shanxi Academy of Medical Sciences, Tongji Shanxi Hospital, Taiyuan, Shanxi, 030032, People's Republic of China.

Journal of inflammation research

|July 23, 2025

概括

乳酪蛋白 (LTF) 和蛋白3 (PRTN3) 是毒症相关的急性呼吸困扰综合征 (ARDS) 的关键基因,与中性粒细胞外细胞陷 (NET) 相关. 这些基因显示出诊断潜力,并可能作为ARDS的治疗点.

科学领域:

基因组学就是基因组学.
生物信息学是一种生物信息学.
分子生物学分子生物学

背景情况:

与败血症相关的急性呼吸困扰综合征 (ARDS) 提出了重大的临床挑战.
中性细胞外细胞陷 (NETs) 在与败血症相关的ARDS的发病过程中发挥着至关重要的作用.
识别特定的分子标记物对于改善诊断和治疗至关重要.

研究的目的:

为了确定关键的基因与中性细胞外细胞陷 (NETs) 相关的毒相关的ARDS.
探索已识别的基因的诊断和治疗潜力.
为了研究潜在的小分子抑制剂用于向治疗.

主要方法:

在GSE32707数据集上利用生物信息学分析来识别差异表达基因 (DEG).
执行了权重基因共同表达网络分析 (WGCNA) 和机器学习算法来确定关键基因.
通过RT-qPCR与NETs基因组进行交叉引用的DEG和验证的枢纽基因表达.
采用分子对接来预测潜在的治疗化合物针对已识别的枢纽基因.

主要成果:

在与败血症相关的ARDS模块中确定了485个DEG和332个常见基因.
确定LTF和PRTN3是具有显著诊断潜力的关键枢纽基因.
通过RT-qPCR检测证实了血症相关ARDS患者中LTF和PRTN3的显著上调.
尼梅苏利德和米诺环林被预测为潜在的治疗剂,准这些枢纽基因.

结论:

已确定LTF和PRTN3是败血症相关ARDS中关键的NETs相关基因.
这些基因显示出作为诊断生物标志物的前景.
LTF和PRTN3代表了与败血症相关的ARDS的潜在治疗点.

关键词:

在 ARDS 中,ARDS 是指 ARDS 的类型.生物信息学是一种生物信息学.中性粒细胞外细胞陷败血症是什么败血症是什么

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