OMNIgene.SPUTUM用于在环境温度下运输唾液进行结核检测:孟加拉国经验
Senjuti Kabir1, Sabrina Choudhury1, Mohammad Khaja Mafij Uddin1
1Infectious Diseases Division, icddr,b, Mohakhali, Dhaka, Bangladesh.
概括
OMNIgene.SPUTUM (OM-S) 是一种安全有效的方法来运输用于结核病检测的唾液. 它确保了涂抹显微镜,培养和Xpert MTB/RIF的可靠结果,即使是在远程医疗机构.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 诊断技术 诊断技术 诊断技术
背景情况:
- 唾液的运输对于诊断结核病 (TB) 是至关重要的.
- 评估新的样本保存和运输系统对于改善结核病诊断至关重要,特别是在资源有限的环境中.
- OMNIgene.SPUTUM (OM-S) 是一个旨在稳定和运输唾液样本的系统.
研究的目的:
- 为了评估OMNIgene.SPUTUM (OM-S) 对于唾液运输的性能.
- 为了比较使用OM-S运输的唾液样本的诊断测试结果与传统方法.
- 评估OM-S在涂抹显微镜,培养和Xpert MTB/RIF测试方面的可靠性.
主要方法:
- 在达卡进行了一项探索性研究,涉及444名涂抹阳性肺结核患者.
- 唾液样本被分为"OM-S未处理"和"OM-S处理"部分.
- 样品被运送到中央实验室进行涂抹显微镜,培养和Xpert MTB/RIF (Xpert) 测试,并进行部分和部位之间的比较.
主要成果:
- 测试结果可用于现场附近样本的OM-S部分之间进行比较.
- 对于远程样本,涂抹显微镜阳性在"OM-S治疗"部分略有下降 (4.1%).
- 埃克斯珀特显示100%的一致性,在"OM-S治疗"部分 (92.3%与89.4%) 的培养阳性更高,污染率明显较低 (2.0%与9.8%).
结论:
- OMNIgene.SPUTUM (OM-S) 是安全的液运输.
- 在OM-S中稳定的唾液可以使用Xpert和培养方法可靠地测试.
- 建议进行进一步的研究以验证这些发现并评估成本效益.
相关概念视频
01:28Pulmonary Tuberculosis V
241
Medical management of tuberculosis (TB) patients involves a comprehensive approach that includes diagnosis, treatment, and monitoring. The specific strategies can vary depending on the type of tuberculosis (latent or active), the patient's overall health status, and other considerations.
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
Latent tuberculosis infection occurs when TB bacteria are present in a person's body, but are not causing illness or symptoms. It is not contagious, and preventive treatment is crucial to avoid the...
241
01:20Sputum Studies II: Culture and Sensitivity
598
Description
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
Sputum culture and sensitivity is a medical procedure used to diagnose bacterial infections in the respiratory tract and select the most appropriate antibiotics for treatment. This process involves analyzing sputum samples of thick and opaque secretions produced in the lungs and airways. These samples are collected from patients and then sent to the laboratory for analysis.
The test can identify various pathogens responsible for respiratory infections, including Streptococcus,...
598
01:26Pulmonary Tuberculosis IV
207
Tuberculosis, more commonly referred to as TB, is an infectious disease stemming from Mycobacterium tuberculosis. While it primarily impacts the lungs, TB can also affect other body areas. Given its severity and global impact, timely and accurate diagnosis is crucial for controlling its spread and improving patient outcomes.
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
Several diagnostic approaches are used to detect TB. The conventional method is the Tuberculin Skin Test (TST), also known as the Mantoux test. However, this method has...
207
01:26Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture
294
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
294
01:29Pulmonary Tuberculosis I
329
Tuberculosis, often called TB, is a contagious illness primarily caused by Mycobacterium tuberculosis. It mainly affects the lung parenchyma but can also impact other body parts.
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
Causative Organism
The primary infectious agent causing tuberculosis is Mycobacterium tuberculosis, a slow-growing, acid-fast, aerobic rod that exhibits sensitivity to heat and ultraviolet light. Instances of Mycobacterium bovis and Mycobacterium avium contributing to the development of TB infection are rare.
Mode of...
329
01:28Pulmonary Tuberculosis II
371
Tuberculosis, or TB, is a bacterial infectious disease caused by Mycobacterium tuberculosis. While its primary impact is on the lungs, leading to pulmonary tuberculosis, it can also affect various other organs, a condition referred to as extrapulmonary tuberculosis.
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
Here is a detailed explanation of its pathophysiology:
Transmission: The process begins when a person inhales droplet nuclei containing M. tuberculosis. These are typically released into the air when an individual with pulmonary or...
371