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Updated: Sep 14, 2025

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Establishment of an Extracellular Acidic pH Culture System
Published on: November 19, 2017
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微环境pH调节对结直肠癌细胞的作用 - 一项初步研究
J Basak1, M Wlodarczyk1, K Marynczak1
1Department of General and Oncological Surgery, Faculty of Medicine, Medical University of Lodz, Lodz, Poland.
概括
性pH对结肠直肠癌 (CRC) 细胞的影响不同;HT-29细胞显示活力增加,而Caco-2细胞减少. 基因表达的变化为细胞对pH值变化的反应提供了洞察力.
科学领域:
- 在瘤学瘤学.
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 酸性瘤外表和性内部的pH梯度逆转是癌细胞的标志.
- 这种pH不平衡会影响瘤生长,转移和治疗疗效.
- 了解细胞对改变pH值的反应对于开发有效的癌症治疗至关重要.
研究的目的:
- 研究性pH对结直肠癌 (CRC) 细胞系 (Caco-2 和 Ht-29) 活力的影响.
- 分析关键基因 (BAX,MKI67,GPR4) 的表达水平,以应对不同的pH条件.
- 使用正常人体纤维细胞 (BJ-CRL-2522) 作为对照对比分析.
主要方法:
- 在性pH条件下,对Caco-2,Ht-29和BJ-CRL-2522细胞系进行了细胞活力测试.
- 对BAX,MKI67和GPR4的基因表达分析使用RT-qPCR进行.
- 在不同的pH条件下观察到细胞的形态变化.
主要成果:
- 在性pH值下,HT-29细胞表现出增加的生存能力和形态变化,而Caco-2细胞在pH值超过9时表现出降低的生存能力.
- 在性条件下,BAX和GPR4的表达在Ht-29和BJ细胞中被上调.
- 在HT-29和BJ细胞中,MKI67的表达略有增加,但在Caco-2细胞中下降,在Caco-2细胞中观察到基因表达的显著变化.
结论:
- 化不同影响结直肠癌细胞活力和基因表达.
- 这项研究提供了关于化下细胞过程的新见解,但强调了需要进一步研究的需要.
- 化在CRC治疗中的确切作用仍需确定,需要进行额外的研究.
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