Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Regulation of Metabolism01:19

Regulation of Metabolism

9.9K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
9.9K
Regulated Protein Degradation02:58

Regulated Protein Degradation

7.6K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.6K
Feedback Inhibition00:46

Feedback Inhibition

54.6K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
54.6K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

8.8K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.8K
Allosteric Regulation01:08

Allosteric Regulation

59.1K
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
59.1K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.0K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.0K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Thrombin-conjugated cationic nanogels loaded with CpG enhance tumor vessel infarction by interpenetrating double gel network and immune activation.

Colloids and surfaces. B, Biointerfaces·2026
Same author

Aspirin prevents postoperative peritoneal adhesions by inhibiting the TGF‑β1/Smad signaling pathway in rats.

Molecular medicine reports·2026
Same author

4-hydroxy-5-methyl-3(2H)-furanone mediates widespread interkingdom communications between fungi and prokaryotes.

Genome biology·2026
Same author

Small-Target Ship Detection with Joint Spatio-Temporal Features Across Multiple Frames.

Sensors (Basel, Switzerland)·2026
Same author

Advances in Magnetic Nanomaterials, Ferrofluids, and Ferrogels: From Structure to Biomedical and Engineering Applications.

Gels (Basel, Switzerland)·2026
Same author

TCP21 negatively regulates photomorphogenesis by integrating phyB- and HY5-mediated signaling pathways in Arabidopsis.

The Plant journal : for cell and molecular biology·2026

相关实验视频

Updated: Sep 14, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.2K

通过通过相分离介导的微聚合物调节酶活性.

Yang Wang1,2,3, Juzheng Yuan3,4, Niu Dai3,4

  • 1Department of Hepatology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, 710004, P. R. China.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|July 23, 2025
PubMed
概括

研究人员开发了D-介导的微聚合物降解 (DPMD) 以通过诱导酶的液固相分离 (LSPS) 来治疗术后胰腺 (POPF). 这种新的方法有效地隔离了致病酶,减少了炎症,并在临床前模型中改善了生存率.

关键词:
这是一种性类.酶分离酶的分离过程微聚合物是一种微聚合物.阶段分离的阶段分离.手术后的胰腺.

更多相关视频

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.5K
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
06:49

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

3.0K

相关实验视频

Last Updated: Sep 14, 2025

Chemical Dimerization-Induced Protein Condensates on Telomeres
08:52

Chemical Dimerization-Induced Protein Condensates on Telomeres

Published on: April 12, 2021

3.2K
Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation
08:27

Aqueous Droplets Used as Enzymatic Microreactors and Their Electromagnetic Actuation

Published on: August 28, 2017

5.5K
Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans
06:49

Monitoring Protein Aggregation Kinetics In Vivo using Automated Inclusion Counting in Caenorhabditis elegans

Published on: December 17, 2021

3.0K

科学领域:

  • 生物化学和分子生物学
  • 生物材料科学 生物材料科学
  • 翻译医学是一种翻译医学.

背景情况:

  • 液态固态分离 (LSPS) 是形成影响生化反应的聚合物的关键生物分子过程.
  • 手术后胰腺 (POPF) 是一种严重的并发症,其特点是血清皮质体中的病原性酶活性.
  • 目前对POPF的治疗方法缺乏向的酶封存策略.

研究的目的:

  • 开发一种使用LSPS治疗POPF的新型酶调节策略.
  • 创建一个D-介导的微聚合物降解 (DPMD) 系统,用于捕获素和化学素.
  • 在临床前POPF模型中评估DPMD的疗效和安全性.

主要方法:

  • 设计的DPMD具有特定的基因,用于结合阴离子和疏水性残留物,促进β片形成和自我组装.
  • 诱导LSPS形成酶微聚合物,从细胞外环境中隔离目标蛋白酶.
  • 在大鼠POPF模型中评估DPMD的疗效,测量胰腺液体泄漏,炎症标志物,存活率,并进行毒性评估.

主要成果:

  • DPMD成功诱导了LSPS,形成了捕获目标酶 (trypsin和 chymotrypsin) 的微聚合物.
  • 在大鼠POPF模型中,DPMD显著降低了胰腺液体泄漏和炎症标志物,从而改善了生存率.
  • 毒性研究表明,DPMD在超治疗剂量下耐受性良好,显示出良好的安全性.

结论:

  • 在POPF治疗中,DPMD通过利用LSPS用于病原性酶封存,代表了显著的翻译进步.
  • DPMD系统有效地隔离和清除有害酶,提供了一个有前途的治疗策略.
  • 这种方法扩大了LSPS在治疗各种酶介导疾病中的潜在应用.