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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

9.6K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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Southern Blot02:57

Southern Blot

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Agarose gel electrophoresis is very useful in separating DNA fragments by size. Running a DNA ladder containing fragments of the known length alongside the sample helps determine the approximate length of the sample DNA fragments. However, additional steps are needed to verify the sequence identity of the sample DNA fragments.
Denatured DNA fragments must be transferred onto a carrier membrane from the gel to make it accessible to a probe - a small ssDNA fragment complementary to the target DNA...
20.2K
Labeling DNA Probes03:31

Labeling DNA Probes

8.4K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.4K

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相关实验视频

Updated: Sep 14, 2025

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

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使用生物化反义探针对特定转录的隔离.

Antoine Praité1, Laurent Delpy2

  • 1UMR CNRS 7276, Inserm 1262, Université de Limoges, Limoges, France; CRIBL lab (Control of the B-cell Immune Response and Lymphoproliferations), Limoges, France; Team 3, BioPIC (Biology of Plasma Cells, Immunopathology and Cancer), Limoges, France.

Methods in molecular biology (Clifton, N.J.)
|July 23, 2025
PubMed
概括

这项研究引入了一种新的方法,用于使用生物化DNA探针分离特定的RNA分子. 这种技术增强了对基因表达调节和RNA修饰的研究,具有高特异性.

关键词:
生物化探头探头捕获RNA 捕获RNA的过程在RNA下拉式下拉式.转录内容丰富 转录内容丰富转录分离隔离 转录分离

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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Novel RNA-Binding Proteins Isolation by the RaPID Methodology

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相关实验视频

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An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

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科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • RNA分子对于基因表达调节至关重要,与蛋白质相互作用并进行修改.
  • 研究单个RNA转录需要具有最小偏差的选择性隔离方法.
  • 全球RNA丰富方法往往缺乏对转录特异性分析的分辨率.

研究的目的:

  • 描述一种基于杂交的新型协议,用于选择性RNA隔离.
  • 为了实现特定RNA分子的向丰富.
  • 促进对RNA结合蛋白和RNA修饰的研究.

主要方法:

  • 开发使用生物化反感 DNA 探针进行基于杂交的 RNA 捕获的协议.
  • 关键步骤的详细概述:探头设计,杂交和净化.
  • 专注于在RNA分离中实现高特异性和可重复性.

主要成果:

  • 该协议允许对特定RNA转录的选择性隔离.
  • 对于研究转录关联RNA结合蛋白 (RBPome) 的证明有用.
  • 能够对RNA修饰和转录后调节进行精细分析.

结论:

  • 描述的协议提供了一个针对特定RNA分离的有针对性的方法.
  • 这种方法为转录后基因调节提供了精细的视角.
  • 它是研究RNA-蛋白相互作用和修改的宝贵工具.