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相关概念视频

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

258
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
258
CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Homologous Recombination02:31

Homologous Recombination

52.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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相关实验视频

Updated: Sep 14, 2025

CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

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克里斯普尔-Cas9及其生物信息学工具:系统性审查

Alicja Jasieniecka1, Inês Domingues1,2

  • 1Polytechnic University of Coimbra, Rua da Misericórdia, Lagar dos Cortiços, S. Martinho do Bispo, 3045-093 Coimbra, Portugal.

Current issues in molecular biology
|July 23, 2025
PubMed
概括

生物信息学工具对CRISPR-Cas9遗传研究至关重要,有助于指导RNA设计和数据分析. 本次审查强调了需要集成的多任务平台来简化复杂的CRISPR工作流程.

关键词:
克里斯普尔是什么意思?克里斯普尔是什么意思?转基因生物工具 转基因生物工具生物信息学是一种生物信息学.

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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相关实验视频

Last Updated: Sep 14, 2025

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Published on: November 1, 2024

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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

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科学领域:

  • 遗传学 是一个遗传学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 克里斯普尔-Cas9技术改变了基因研究.
  • 生物信息学工具对于CRISPR应用是不可或缺的,包括指导RNA设计,目标外预测和数据分析.
  • 进行了系统审查,以评估CRISPR-Cas9研究的现有生物信息学工具.

研究的目的:

  • 系统地审查和总结在CRISPR-Cas9研究中使用的生物信息学工具的功能和关键特性.
  • 识别常用的工具及其主要应用.
  • 突出局限性,并建议工具开发的未来方向.

主要方法:

  • 在PubMed,Google Scholar和其他数据库中进行系统的文献搜索.
  • 纳入标准:2012年以后发表的研究.
  • 最终搜索于2024年11月9日进行.

主要成果:

  • 七项研究符合纳入标准,描述了大约45种生物信息学工具.
  • 常见的突出工具包括CRISPResso,CHOPCHOP和Cas-OFFinder,重点是单导向RNA (sgRNA) 设计.
  • 大多数工具都针对特定的任务,经常缺乏实验验证,有些工具表现出潜在的作者偏见.

结论:

  • 需要综合的多任务生物信息平台来简化CRISPR研究工作流程.
  • 未来的工具开发应该优先考虑全面的功能和可访问性.
  • 标准化和实验验证的平台对于推进CRISPR-Cas9研究至关重要.