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克里斯普尔-Cas9及其生物信息学工具:系统性审查

Alicja Jasieniecka¹, Inês Domingues^1,2

¹Polytechnic University of Coimbra, Rua da Misericórdia, Lagar dos Cortiços, S. Martinho do Bispo, 3045-093 Coimbra, Portugal.

Current issues in molecular biology

|July 23, 2025

概括

生物信息学工具对CRISPR-Cas9遗传研究至关重要,有助于指导RNA设计和数据分析. 本次审查强调了需要集成的多任务平台来简化复杂的CRISPR工作流程.

科学领域:

遗传学是一个遗传学.
生物信息学是一种生物信息学.
分子生物学分子生物学

背景情况:

克里斯普尔-Cas9技术改变了基因研究.
生物信息学工具对于CRISPR应用是不可或缺的,包括指导RNA设计,目标外预测和数据分析.
进行了系统审查,以评估CRISPR-Cas9研究的现有生物信息学工具.

研究的目的:

系统地审查和总结在CRISPR-Cas9研究中使用的生物信息学工具的功能和关键特性.
识别常用的工具及其主要应用.
突出局限性,并建议工具开发的未来方向.

主要方法:

在PubMed,Google Scholar和其他数据库中进行系统的文献搜索.
纳入标准:2012年以后发表的研究.
最终搜索于2024年11月9日进行.

主要成果:

七项研究符合纳入标准,描述了大约45种生物信息学工具.
常见的突出工具包括CRISPResso,CHOPCHOP和Cas-OFFinder,重点是单导向RNA (sgRNA) 设计.
大多数工具都针对特定的任务,经常缺乏实验验证,有些工具表现出潜在的作者偏见.

结论:

需要综合的多任务生物信息平台来简化CRISPR研究工作流程.
未来的工具开发应该优先考虑全面的功能和可访问性.
标准化和实验验证的平台对于推进CRISPR-Cas9研究至关重要.

关键词:

克里斯普尔是什么意思?克里斯普尔是什么意思?转基因生物工具转基因生物工具生物信息学是一种生物信息学.

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