在成像,电生理学和光遗传学的小鼠中合成,植入和使用聚二甲基西洛作为头骨替代剂的协议

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Kengo Takahashi¹, Gerjan Huis In 't Veld², Davide Benedetti³

¹Swammerdam Institute for Life Sciences, University of Amsterdam, 1098XH Amsterdam, the Netherlands; Amsterdam Brain and Cognition, University of Amsterdam, 1098XH Amsterdam, the Netherlands.

|July 23, 2025

概括

研究人员开发了一种新方法,使用聚甲基 (PDMS) 窗口对小鼠进行慢性脑部成像. 这种技术允许光学和机械访问,改善神经元活动研究.

科学领域:

神经科学是一个神经科学.
生物医学工程生物医学工程
材料科学材料科学材料科学

背景情况:

记录和操纵神经元活动的先进技术对于理解大脑功能至关重要.
现有的方法往往缺乏对大脑的联合光学和机械访问,限制了研究范围.

研究的目的:

提出一个创建和利用聚甲基 (PDMS) 窗户作为头骨替代品的协议.
为了使大脑访问改善的小鼠能够进行慢性广场和两光子成像.

主要方法:

合成,植入和使用PDMS窗口作为头骨替代品的协议.
病毒注射和多部位探头植入的详细步骤.
在老鼠模型中,慢性脑部接入的手术程序.

主要成果:

成功实施PDMS窗口用于慢性光学成像.
结合成像与其他神经科学技术的可行性.
PDMS窗口为长期的大脑研究提供了强大的和生物相容的接口.

结论:

PDMS窗口为光学和机械脑接入的组合提供了一个有效的解决方案.
该协议促进了对小鼠神经元活动的先进纵向研究.
开发的方法增强了神经科学研究的能力,需要深入进入大脑.

关键词:

行为行为行为.认知神经科学认知神经科学显微镜的使用方法神经科学是一个神经科学.

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