单色光对肉肠干细胞自我更新和分化的影响
Xinlei Tao1, Ge Hu2, Zixu Wang1
1Laboratory of Anatomy of Domestic Animal, State Key Laboratory of Veterinary Public Health and Safety, College of Veterinary Medicine, China Agricultural University, Beijing, 100193, China.
Poultry science
|July 23, 2025
概括
蓝光暴露显著提高肉生长和肠道健康,通过促进肠道干细胞的自我更新和分化. 这通过Wnt/β-catenin和Notch信号通路增强了肠道形态和功能.
科学领域:
- 动物科学动物科学
- 细胞生物学 细胞生物学
- 生理学 生理学 生理学
背景情况:
- 肠道稳态是由肠道干细胞 (ISC) 维持的.
- 了解影响ISC自我更新和差异化的因素对于肉发展至关重要.
- 单色光对鸟类肠道健康的影响需要进一步调查.
研究的目的:
- 研究单色光,特别是蓝光 (BL) 对肉ISC自我更新和差异化的影响.
- 阐明BL影响肉生长,肠道形态和功能的机制.
- 评估Wnt/β-catenin和Notch信号通路在BL介导效应中的作用.
主要方法:
- 科布500肉从化后的0日到35日暴露在白色,红色,绿色或蓝色光线下.
- 测量了身体重量,状状体的长度,密室深度和状体与密室的比率.
- 分析了与营养吸收,细胞分化和信号通路相关的关键基因和蛋白质的mRNA和蛋白质水平.
- 免疫组织化学被用来评估细胞增殖标志物.
主要成果:
- 暴露在蓝光下的肉体重量显著增加,状毛长度增加,毛与密室比率有所改善.
- 蓝光显著增加了参与营养物质运输 (Sglt1,Glut1,Slc7a5,Fabp2),免疫反应 (sIgA) 和细胞分化 (Tff3) 的基因表达.
- 蓝光增强了肠细胞,杯细胞,帕内斯细胞和肠内分泌细胞的增殖和分化,激活了Wnt/β-catenin和Notch信号通路.
结论:
- 蓝光暴露对肉生长和肠道发育产生积极影响.
- 蓝光促进肠道干细胞的自我更新和分化,增强肠道形态和功能.
- Wnt/β-catenin和Notch信号通路是蓝光对肉肠道有益影响的关键媒介.
更多相关视频
09:10Intestinal Epithelial Regeneration in Response to Ionizing Irradiation
Published on: July 27, 2022
2.3K
10:05Stimulation of Stem Cell Niches and Tissue Regeneration in Mouse Skin by Switchable Protoporphyrin IX-Dependent Photogeneration of Reactive Oxygen Species In Situ
Published on: May 8, 2020
2.0K
相关概念视频
Renewal of Intestinal Stem Cells
2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
Role Of Notch Signalling In Intestinal Stem Cell Renewal
2.2K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Adult Stem Cells
30.6K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
30.6K
Maintenance of the ES Cell State
2.3K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.3K
