多方面的Sacha Inchi (Plukenetia volubilis L.) 蛋白质:从分子组成到提取技术,技术功能性质和食品应用
Fatema Hossain Brishti1, Wan Zunairah Wan Ibadullah2, Mohamad Ariff Hanafi2
1Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Food chemistry
|July 23, 2025
概括
沙沙仁慈种子提供了一个可持续的,高蛋白质的食物来源,含有所有必需的氨基酸. 优化提取和水解解释放了它们的潜力,用于营养药物应用和改进食品产品.
科学领域:
- 食品科学 食品科学 食品科学
- 营养生物化学 营养生物化学
- 可持续农业 可持续农业
背景情况:
- 沙沙 (Plukenetia volubilis) 种子被公认为一种有价值的,可持续的蛋白质来源.
- 种子含有相当多的蛋白质含量,从24%到33%不等,油压副产品高达62%.
- 蛋白质分离物显示的分子重量分布在8-75 kDa之间,显著的波段在20-50 kDa范围内.
研究的目的:
- 为了审查Sacha Inchi蛋白质的营养特征.
- 探索提取条件对蛋白质特性的影响.
- 评估Sacha Inchi水解剂在营养药应用中的潜力.
主要方法:
- 氨基酸分析以确定必需氨基酸 (EAA) 含量.
- 在不同的pH条件下 (例如pH11) 提取蛋白质,以评估产量和可溶性.
- 研究联合提取技术,包括超声波和酶辅助.
- 优化水解过程以产生生物活性.
主要成果:
- 沙沙仁花蛋白含有所有EAA,其中histidine,tryptophan和valine特别丰富.
- 根据世卫组织针对特定年龄组的标准,氨酸和氨酸被确定为潜在的限制性氨基酸.
- 在pH 11下提取增加了蛋白质产量,但导致了变质和降低溶解度.
- 超声波和酶辅助提取改善了蛋白质的产量和纯度.
- 优化的水解产生了具有抗氧化,抗高血压和抗糖尿病功能的Sacha Inchi.
结论:
- 沙沙仁芝种子代表了一种多功能和高质量的蛋白质资源.
- 定制提取pH值和采用超声波或酶辅助等先进技术对于优化蛋白质产量和功能至关重要.
- 沙沙仁芝蛋白质的水解产生具有重要的营养潜力的生物活性,用于食品和健康应用.
相关概念视频
Proteins: Dietary Sources and Requirements
645
Consuming animal-based products offers high-quality proteins that contain optimal levels and combinations of essential amino acids, crucial for tissue repair and growth. Foods like eggs, milk, fish, and most meats are a source of complete proteins. Legumes and cereals are abundant in proteins; however, they typically lack a full range of essential amino acids. As a result, they are considered incomplete protein sources. Some plant sources like soybeans, quinoa, and amaranth do contain complete...
645
Mechanical Protein Function
2.1K
2.1K
Mechanical Protein Functions
5.1K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force.
5.1K
Protein Complexes with Interchangeable Parts
1.9K
1.9K
Tagging and Fusion Proteins
6.9K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.9K
Protein Modifications in the RER
5.6K
Modification of secretory and transmembrane proteins entering the rough ER begins in the ER lumen. These modifications aid in protein folding and stabilize the acquired tertiary structure. Protein modifications in the rough ER co-occur at different stages of protein folding.
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal...
5.6K


