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来自同一个呼出的呼吸凝析样本的挥发性有机化合物和蛋白质组学数据

Shannon E Schrader¹, Joshua R Hansen¹, Isabelle O'Bryon¹

¹Chemical and Biological Signatures Group, Pacific Northwest National Laboratory, Richland, WA 99352, United States of America.

Journal of breath research

|July 23, 2025

概括

从单个呼气凝结物 (EBC) 样本中分析挥发性有机化合物 (VOC) 和蛋白质,简化了诊断. 对VOC的薄膜固体相微提取 (TF-SPME) 没有影响EBC中的蛋白质检测.

科学领域:

生物标志物发现发现
分析化学分析化学
多个omics的多个omics.

背景情况:

呼出的呼吸凝聚物 (EBC) 是一种用于疾病诊断的非侵入性来源.
从单个EBC样本中分析多个分析剂简化了诊断过程.
目前的方法通常需要单独的样本进行不同的分析,增加了患者的负担.

研究的目的:

调查从同一EBC样本中分析挥发性有机化合物 (VOC) 和蛋白质的可行性.
确定用于VOC分析的薄膜固体相微提取 (TF-SPME) 是否会影响随后的蛋白质组分析.
从单个样本提前进行多omics呼吸分析.

主要方法:

收集了EBC样本,并分为两组:TF-SPME用于VOC分析,非TF-SPME用于蛋白质组分析.
使用二维气体染色学质谱法 (GC x GC-MS) 分析了VOC.
蛋白质被分析使用液态色谱与双重质谱 (LC-MS/MS).

主要成果:

与对照组相比,EBC样本中发现184种挥发性有机化合物比对照组更丰富.
在TF-SPME和非TF-SPME组之间检测到的蛋白质数量没有显著差异.
在两种样本类型中检测到206种独特蛋白质中的144种,表明TF-SPME不会阻碍蛋白质组分析.

结论:

TF-SPME是一种适合于VOC分析的方法,而不会损害EBC中的蛋白质组完整性.
这项研究支持从单个EBC样本开发多组体分析.
这种方法提供了一种更有效,更为患者友好的诊断策略.

关键词:

呼出的呼吸凝结物是空气中的凝结物.液态染色学-双重质谱学质谱学蛋白质组学蛋白质组学二维气体染色学-质谱学二维气体染色学挥发性有机化合物挥发性有机化合物

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