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Opt1进口的CoA前体是作为谷氨混合二硫化物中的谷氨

Jouke Jan Wedman¹, Lotte de Vries¹, Bart van Lingen¹

¹Department of Biomedical Sciences, University of Groningen, University Medical Center Groningen, Groningen, the Netherlands.

The Journal of biological chemistry

|July 23, 2025

概括

研究人员发现了一种新的合作酶A (CoA) 生产途径,使用素 (PanSH) 或4'-素素素 (PPanSH) 通过寡载体Opt1.1. 这一发现为治疗COA相关疾病和控制微生物生长提供了新的策略.

科学领域:

生物化学生物化学
分子生物学分子生物学
细胞生物学细胞生物学

背景情况:

辅酶A (CoA) 对于细胞内生物合成至关重要,主要通过需要泛氨酸,ATP和氨酸的正规途径进行合成.
存在其他可替代的CoA生物合成途径,利用泛氨酸 (PanSH) 或4-酸氨酸 (PPanSH) 作为前体,特别是在单细胞生物中.
对于PanSH和PPanSH的细胞吸收机制在很大程度上仍然没有特征,不像托酸转运体.

研究的目的:

阐明PanSH和PPanSH的细胞吸收机制.
确定和描述一种非正规的协酶A (CoA) 生物合成途径.
探索这种途径对微生物生长和人类疾病的影响.

主要方法:

在体内实验中利用酵母遗传学的实验.
使用化学可追溯化合物来监测吸收和新陈代谢.
研究细胞外二硫化物形成和转运器介导吸收.

主要成果:

在细胞外,PanSH和PPanSH与谷氨形成混合二硫化物.
这些二硫化物通过寡聚载体Opt1.1输入细胞.
进口的PanSH/PPanSH被转化为CoA,使正规通路蛋白变得无用,并在低氨酸条件下赋予生长优势.

结论:

已经确定了一种新的,非正规的CoA生物合成途径,涉及Opt1介导的PanSH/PPanSH二硫化物吸收.
这种途径绕过了对正规的CoA生物合成成分的需求,特别是在营养有限的条件下.
这些发现为开发治疗COA相关疾病和操纵微生物种群的治疗提供了基础.

关键词:

辅酶A 是一种代谢代谢代谢代谢子子子子这是一个屏幕屏幕屏幕屏幕屏幕屏幕.酵母酵母是一种酵母.

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