Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.4K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.4K
Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.3K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.3K
Catenins01:23

Catenins

2.4K
Catenins are characterized by multiple binding domains and dynamic structures that allow them to function as linker proteins in cell junction complexes. All catenins, except α-catenin, contain a characteristic protein sequence called the armadillo repeat and are therefore also called armadillo proteins.
Catenins in Cell Junctions
Catenins bind to cell adhesion molecules such as cadherins and link them to different cytoskeletal proteins depending on the type of cell junction. At the...
2.4K
Feedback Regulation of Calcium Concentration01:27

Feedback Regulation of Calcium Concentration

3.5K
Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
3.5K
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

8.7K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
8.7K
Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

8.6K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Expert opinion-based recommendations promoted by GTIPO-SEDAR to optimize the therapeutic management of intra-abdominal candidiasis.

Revista espanola de anestesiologia y reanimacion·2025
Same author

[Translated article] Expert Recommendations on Topical Therapy for Psoriasis from the Spanish Psoriasis Working Group (GPS).

Actas dermo-sifiliograficas·2025
Same author

Expert Recommendations on Topical Therapy for Psoriasis from the Spanish Psoriasis Working Group (GPS).

Actas dermo-sifiliograficas·2025
Same author

Tuning the aspect ratio of silver nanospheroids embedded in silica: erratum.

Optics letters·2023
Same author

Impact of Aspergillus spp. isolation in the first 24 hours of admission in critically ill patients with severe influenza virus pneumonia.

Medicina intensiva·2022
Same author

Management of infectious complications associated with coronavirus infection in severe patients admitted to ICU.

Medicina intensiva·2021

相关实验视频

Updated: Sep 14, 2025

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

1.6K

无处不在的calpains在脂肪细胞分化过程中表现出差异动态和功能.

L Rodríguez-Fernández1, R Zaragozá2, J R Viña3

  • 1Departamento de Bioquímica y Biología Molecular, Facultad de Medicina. Universidad de Valencia, Spain.

Archives of biochemistry and biophysics
|July 23, 2025
PubMed
概括

卡尔帕因,依赖的蛋白酶,对于脂肪生成至关重要. 这项研究揭示calpain-1 (CAPN1) 裂解基因组H3,使细胞周期退出和脂肪细胞分化成为可能,突出显示了代谢障碍的治疗潜力.

关键词:
3T3-L1区分方式卡尔帕因-2是一种疼痛.裂口 裂口 裂口 裂口基因组 H3 基因组 H3核卡尔派因-1-1的核反应器

更多相关视频

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.3K
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

3.0K

相关实验视频

Last Updated: Sep 14, 2025

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
04:46

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Published on: February 3, 2023

1.6K
Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis
08:34

Mechanism of Regulation of Adipocyte Numbers in Adult Organisms Through Differentiation and Apoptosis Homeostasis

Published on: June 3, 2016

15.3K
Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
09:41

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro

Published on: March 17, 2023

3.0K

科学领域:

  • 生物化学和分子生物学
  • 细胞生物学 细胞生物学
  • 代谢研究研究 代谢研究

背景情况:

  • 脂肪组织的平衡至关重要,与内分泌功能障碍和疾病相关的干扰.
  • 卡尔帕因是影响脂肪生成的依赖蛋白酶,但异形特异性的作用尚不清楚.
  • 了解calpain功能是解决肥胖和代谢障碍的关键.

研究的目的:

  • 研究calpain-1 (CAPN1) 和calpain-2 (CAPN2) 在3T3-L1前脂肪细胞分化中的异型和相位特异性的作用.
  • 阐明calpains调节脂肪生成的分子机制.
  • 确定calpains作为代谢疾病的潜在治疗点.

主要方法:

  • 激素诱导的3T3-L1前细胞的分化.
  • 对calpain异形表达,活性和局部化的分析.
  • 使用siRNA,细胞循环分析和西式涂抹的枯竭研究.
  • 免疫光,近距离结合试验,以及希斯H3裂解试验.

主要成果:

  • 在差异化过程中,CAPN1和CAPN2都被调节和重新分配.
  • 缺少CAPN1或CAPN2受损的细胞周期退出和脂肪细胞分化.
  • 鉴定出CAPN1是主要的异型,它负责希斯H3分裂,这是染色体重塑的一个关键事件.
  • 卡尔帕因抑制阻止了希斯H3裂变,证实了CAPN1的作用.

结论:

  • 卡尔帕因在脂肪生成程序中扮演着关键的,特定阶段的角色.
  • 通过CAPN1介导的希斯H3裂变对于从线粒细胞克隆扩张过渡到早期分化至关重要.
  • 卡尔帕因代表了肥胖和相关代谢障碍的有希望的治疗点.