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在Penaeus vannamei中的指转录因子PvBr-C的双重功能

Zhouyi Shi¹, Lingwei Ruan², Xiuli Chen³

¹College of Marine Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, People's Republic of China; State Key Laboratory Breeding Base of Marine Genetic Resources, Key Laboratory of Marine Genetic Resources of Ministry of Natural Resources, Third Institute of Oceanography, Ministry of Natural Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen, 361005, People's Republic of China.

Fish & shellfish immunology

|July 23, 2025

概括

中的宽复合体 (Br-C) 在发育和免疫方面发挥着双重作用. 这种转录因子调节变和抗病毒防御,但也被白斑综合征病毒 (WSSV) 用于复制.

科学领域:

* 分子生物学 * 分子生物学
* 免疫学免疫学
* 类水产养殖的水产养殖

背景情况:

*宽复合体 (Br-C) 是关节动物发育和变形中的关键转录因子.
* 其在甲动物中的功能,特别是免疫力和病毒相互作用,尚不清楚.
*Penaeus vannamei (太平洋白) 是一个经济重要物种,容易感染白斑综合征病毒 (WSSV).

研究的目的:

* 为了识别和描述Penaeus vannamei.中的Br-C同类物 (PvBr-C).
* 调查PvBr-C在发育,免疫力和WSSV感染中的功能性作用.
* 为了阐明PvBr-C和WSSV复制之间的相互作用.

主要方法:

* 分子克隆和遗传学分析以确定PvBr-C.
*用于功能性研究的组织特异性表达特征和RNA干扰 (RNAi).
*转录组分析和双露西法酶测定用于研究基因调节和病毒相互作用.

主要成果:

* PvBr-C 是 Br-C 的同源,在 P. vannamei 中被发现具有保留域.
*PvBr-C主要表达在血细胞中,与免疫调节有关.
* 抑制PvBr-C抑制了WSSV复制和改变了免疫和发育基因表达.
* PvBr-C被证明可以激活WSSV促销者,这表明它被病毒选择.

结论:

* PvBr-C 作为的双重调节剂,控制发育过程和抗病毒免疫力.
* WSSV劫持了PvBr-C以促进其自身的复制,突出了一个前期的角色.
* 结果提供了关于甲动物变,免疫力和宿主病毒相互作用的见解,这与WSSV疫情管理有关.

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