在法医应用中,使用一次性,基于石墨烯的电化学传感器与单体微固相提取相结合的尿 11-nor-9-carboxy-delta-9-tetrahydrocannabinol 分析的新方法
Kesara Ar-Sanork1, Wichayaporn Kamsong2, Rawisara Woensanthia3
1School of Chemistry, Institute of Science, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima, 30000, Thailand; Institute of Pharmaceutical Analysis, College of Pharmacy/State Key Laboratory of Bioactive Molecules and Druggability Assessment/International Cooperative Laboratory of Traditional Chinese Medicine Modernization and Innovative Drug Development of Ministry of Education (MOE) of China, Jinan University, Guangzhou, 510632, China.
这项研究引入了一种新型的便携式电化学传感器,用于检测尿液中的关键大麻代谢物11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH). 该方法为法医分析提供了传统色谱技术的快速,经济有效和敏感的替代方案.
科学领域:
- 分析化学 分析化学
- 法医科学 法医科学 法医科学
- 电化学 电化学 电化学
背景情况:
- 全球大麻使用量的增加需要快速,便携式的药物检测方法.
- 目前对尿液THC-COOH的量化依赖于复杂而昂贵的染色学技术.
- 需要一种简化,具有成本效益的方法来对法医大麻摄入量进行评估.
研究的目的:
- 开发一种简单,有选择性和敏感的方法来检测人类尿液中的THC-COOH.
- 将单体管管尖固体相提取 (PT-SPE) 与电化学传感器相结合.
- 建立一个便携式和具有成本效益的工具,用于法医大麻测试.
主要方法:
- 在现场合成C18功能化的单立体骨干,用于样品清洁和预度的管管尖内.
- 使用一次性印石墨烯电极 (SPGE) 进行THC-COOH.COOH的电化学检测.
- 系统优化PT-SPE和电化学检测参数.
主要成果:
- 实现了广泛的线性范围 (50-1000 ng/mL),检测极限低 (5.2 ng/mL) 和量化极限低 (17.5 ng/mL).
- 从尿液矩阵中有效隔离THC-COOH,产生14倍的信号增强和良好的恢复 (80-110%).
- 与真实和刺尿样的LC-MS/MS结果有很强的相关性,精度很高 (一天内5.9%,一天内7.1%).
结论:
- 成功开发了第一个用于尿液中THC-COOH测定的电化学传感器.
- 通过将单质PT-SPE与SPGE合,实现THC-COOH的直接检测,从而消除了电极修改.
- 介绍了一种环保,高效,简单和具有成本效益的法医大麻代谢物分析方法.
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