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电子烟和普通香烟诱导心肌细胞的转录和生理变化

Hesham Basma¹, Kajari Dhar², Jason Payne²

¹University of Nebraska Medical Center, Omaha, NE, USA. hbasma@unmc.edu.

BMC cardiovascular disorders

|July 23, 2025

概括

电子烟提取物 (ECE) 和传统香烟提取物 (CSE) 都会损害心脏功能,并改变人类心肌细胞中的基因表达. 这项研究强调了这两种吸烟形式的心脏毒性潜力.

科学领域:

心血管毒理学心血管毒理学
干细胞生物学干细胞生物学
分子心脏病学分子心脏病学

背景情况:

吸烟是众所周知的心脏病的原因之一.
电子烟越来越受欢迎,引发人们对电子烟对心血管的影响的担忧.
这项研究研究了电子烟提取物 (ECE) 和传统香烟提取物 (CSE) 对人体心肌细胞的影响.

研究的目的:

评估人类诱导的多能干细胞衍生心肌细胞 (iPS-CMs) 在暴露于ECE和CSE时的生理和转录变化.
在细胞水平上比较ECE和CSE的心脏毒性作用.

主要方法:

人类iPS-CMs被培养并暴露于ECE和CSE.
使用多电极阵列板 (MEA) 和视频分析 (MuscleMotion) 来测量电气和收缩性能.
用直接数字mRNA检测针对心力衰竭的基因来分析转录资料.

主要成果:

无论是ECE还是CSE都显著延长了心肌细胞节拍周期和到峰值收缩的时间.
同样,ECE和CSE降低了心肌细胞收缩性.
与ECE相比,CSE在与心力衰竭相关的基因的转录形状中引起了更深刻的变化.
基因表达的变化与对 sarcomeric 蛋白质和处理的影响一致.

结论:

人类iPS-CM是研究传统和电子烟毒理学的可行模型.
无论是ECE还是CSE都表现出心脏毒性作用,影响心肌细胞生理和基因表达.
需要进一步的研究来了解与电子烟使用相关的长期心血管风险.

关键词:

电子香烟电子香烟的使用诱导多能干细胞衍生心肌细胞多个电极阵列.肌肉运动肌肉运动

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