相关实验视频
Updated: Sep 14, 2025

16:24
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
Published on: February 21, 2014
20.3K
循环CDYL与 hnRNPL 关联 调节乳腺癌细胞中的 CDYL 异形切换
Serena Bernardi1, Giorgia Risso2, Lorenzo Franchitti3
1Department of Clinical and Biological Sciences, University of Turin, Orbassano, Italy.
Cancer science
|July 24, 2025
概括
循环RNA circCDYL和拼接因子 hnRNPL通过影响替代拼接和促进剂使用,协同调节乳腺癌细胞中的基因表达. 这种相互作用影响关键的细胞过程,并提供潜在的治疗点.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 循环RNAs (circRNAs) 是细胞过程的关键调节者,与癌症相关的失调.
- CircCDYL是一种特定的circRNA,在乳腺癌 (BC) 生物学中发挥作用.
- 了解circCDYL在替代拼接 (AS) 和异形切换中的功能对于BC研究至关重要.
研究的目的:
- 研究circCDYL在调节MCF-7乳腺癌细胞中的替代拼接和异形切换中的作用.
- 确定circCDYL的功能背后的分子机制,包括它与拼接因子的相互作用.
- 阐明circCDYL对基因调节和乳腺癌表观遗传修饰的影响.
主要方法:
- 循环RNA分析和RNA测序以分析基因表达和拼接模式.
- RNA免疫沉和RNA拉下测试以确定蛋白质-RNA相互作用.
- 异形特异性qRT-PCR和表观遗传分析以确认调节机制.
主要成果:
- CircCDYL是MCF-7细胞中最丰富的circRNA,在雌激素受体α (ERα) 沉默时增加.
- 在2900多个替代拼接事件中,circCDYL的 Knockdown显著改变.
- CircCDYL与剪接因子hnrnpl相关,影响AS和替代促进剂使用,观察到表观遗传变化.
结论:
- CircCDYL和hnrnpl在调节乳腺癌细胞中的替代拼接和促进剂使用方面表现出协同作用.
- 这种相互作用涉及CDYL基因促进者的表观遗传修饰.
- 这些发现突出了一个新的监管轴,对乳腺癌的进展和潜在的治疗策略有影响.
相关概念视频
Inhibition of Cdk Activity
4.9K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.9K
lncRNA - Long Non-coding RNAs
8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K
Cadherins in Tissue Organization
3.1K
The cadherins are a superfamily of cell adhesion molecules comprising over 180 variants, with specific tissues expressing a particular combination of cadherin types. Cadherins generally exhibit homophilic binding; i.e., cadherins on one cell bind to cadherins of the same or closely related type on another cell. Thus, cells of the same type have a specific affinity to bind to each other and sort themselves into clusters to form tissues.
Cell Sorting During Development
Cell sorting plays an...
Cell Sorting During Development
Cell sorting plays an...
3.1K
The Nucleolus
9.1K
The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
9.1K
Positive Regulator Molecules
5.7K
Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.
5.7K
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K

