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优化原蛋白生产料和半固体发酵造麦草的过程

Xinyi Li^1,2, Min Li³, Chunyan Liu²

¹Sichuan Province Engineering Technology Research Center of Liquor-Making Grains, Sichuan University of Science & Engineering, No.1 Baita Road, Sangjiang District, Yibin, 644005 Sichuan China.

|July 24, 2025

概括

这项研究开发了一种高蛋白料,来自造草 (BSS),使用特定微生物菌株的顺序发酵. 优化过程显著增加了原始蛋白质含量,提供了可持续的料替代品.

科学领域:

农业科学农业科学
微生物学微生物学
生物技术是生物技术.

背景情况:

酒糖 (BSS) 是一个丰富的农业副产品,具有价值化潜力.
使用BSS的传统方法通常面临蛋白质含量和消化能力的限制.
开发具有成本效益和可持续的方法来提高BSS的营养价值对于动物料生产至关重要.

研究的目的:

开发一种优化的半固体发酵工艺,从BSS.中生产高蛋白料.
评估使用特定微生物菌株进行蛋白质丰富的顺序发酵策略的有效性.
为了提高BSS的碳比率,使用蒸厂的谷物进行增强发酵.

主要方法:

在的帮助下,BSS的高温预处理.
使用*A. niger*进行固体发酵,用于酶生产和茎生物降解.
使用*P. kudriavzevii* 26和*B. subtilis* D3-1.进行液态发酵和繁殖.
使用单因素和直角实验设计优化发酵参数.
在受控的间隔和条件下对微生物菌株进行连续注射.

主要成果:

优化固态发酵条件:BSS与蒸器的粒度比为 4:1,pH值为 4,18% *A. niger* 接种3天.
优化的液态发酵条件:添加2%的尿素,在34°C下连续接种*P. kudriavzevii*26和*B. subtilis*D3-1 (2:1比率,12%的总接种,24小时间隔) 在5天内.
实现了原始蛋白质含量增加1.6倍,从优化后的10.41%增加到18.06%.
通过使用蒸厂的谷物,成功地证明了C/N比率的平衡,减少了对合成的依赖.

结论:

开发的半固体发酵过程有效地将BSS转化为高蛋白料.
使用 *A. niger*, *P. kudriavzevii* 26 和 *B. subtilis* D3-1 的序列发酵是一种可行的蛋白质丰富策略.
蒸谷物的双基质方法提高了营养平衡和发酵效率.
这种方法提供了一种可持续且具有成本效益的方法,可以从农业废物中生产有价值的动物料.

关键词:

造麦,原始蛋白质料,半麦草,原始蛋白质料细胞蛋白质是细胞中的蛋白质.固体固体固体固体国家发酵工艺, 单一的

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