LncRNA TMEM99与IGF2BP2复合起来,以抑制肺腺癌中的自
Zhigang Wu1, Yue Zhao1, Yizhou Peng2
1Department of Thoracic Surgery, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, 200032, China.
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|July 24, 2025
概括
长非编码RNATMEM99在肺腺癌 (LUAD) 中被上调,通过抑制自,促进癌症的进展. 它稳定FUBP3,通过m6A依赖机制增强p21mRNA翻译.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
背景情况:
- 肺腺癌 (LUAD) 的预后不好,长非编码RNA (lncRNAs) 参与了它的进展.
- 在LUAD中lncRNATMEM99的特定作用仍然在很大程度上是未知的,特别是其参与诸如自等细胞过程.
研究的目的:
- 在肺腺癌中研究 lncRNA TMEM99 的生物功能和分子机制.
- 确定TMEM99在调节自的作用及其对LUAD进展的影响.
主要方法:
- 用RNA测序和定量逆转录PCR (qRT-PCR) 来评估TMEM99的表达.
- 在体内和体外测试评估TMEM99对细胞增殖,迁移,入侵和自的作用.
- 涉及蛋白质-RNA相互作用和m6A依赖调节的机制研究.
主要成果:
- 发现TMEM99在LUAD组织和细胞系上升调节,与较差的患者结局相关.
- TMEM99的过度表达促进了LUAD细胞的增殖,迁移和入侵,同时抑制了自.
- TMEM99通过与其KH1和KH4域结合稳定FUBP3蛋白,形成一个复合物,通过IGF2BP2以m6A依赖的方式增强p21mRNA稳定性和翻译.
结论:
- lncRNA TMEM99通过抑制自而在促进LUAD进展方面发挥着重要作用.
- 一种涉及TMEM99,FUBP3,p21mRNA和IGF2BP2 (依赖m6A) 的新型细胞质调节机制有助于LUAD的发病.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K
03:03mTOR Signaling and Cancer Progression
3.9K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
3.9K
02:15Experimental RNAi
6.3K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.3K
01:16TGF - β Signaling Pathway
7.7K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.7K
01:22MicroRNAs
3.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.1K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K