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采矿UDP-Glucosyltransferases开发高效率的生产策略,以获得埃莫丁糖化物

Yongsong Wu^1,2,3,4,5, Ning Xu^2,3,4,5,6, Yingying Xue^2,3,4,5

¹College of Life Science, Dalian Minzu University, Dalian, Liaoning 116600, China.

Journal of agricultural and food chemistry

|July 24, 2025

概括

研究人员开发了一种可持续的微生物平台,用于生产宝贵的制药化合物emodin糖化物. 他们改造了Aspergillus terreus和Escherichia coli,为emodin-6-O-β-d-glucoside (E6G) 和emodin-8-O-β-d-glucoside (E8G) 实现了高标位和转化率.

科学领域:

合成生物学合成生物学
生物技术是生物技术.
酶工程是什么?酶工程是什么?

背景情况:

埃莫丁葡萄糖化物 (E6G和E8G) 显示出药用前景,但由于天然丰度低和复杂的提取,面临生产挑战.
这些化合物的高效生物合成对于它们的大规模可用性至关重要.

研究的目的:

通过合成生物学研究和优化有线真菌中emodin葡萄糖酸的生物合成.
建立一个可持续的微生物生产平台,以生产埃莫丁糖化物.

主要方法:

评估了十个emodin UDP-glucosyltransferases (EUGTs) 并将它们整合到一个emodin积累的Aspergillus terreus菌株中.
在大肠杆菌中开发了一种表达高效EUGT (Ft73BE) 的全细胞催化系统.
对EUGT进行了序列,结构和系遗学分析.

主要成果:

在Aspergillus terreus中,ΔgedA-Ft73BE变异实现了217.5 mg/L的高E6G标位.
埃舍里希亚大肠杆菌的全细胞系统表现出高转化率为78.9%的emodin葡萄糖化.
OsUGT表现出双位点糖化活性,提供了对酶机制的洞察力.

结论:

这项研究为大规模生产埃莫丁葡萄糖化物建立了一个强大而可持续的平台.
这些发现有助于更好地理解微生物宿主中埃莫丁糖化和异质植物蛋白表达的过程.

关键词:

这是一种Aspergillus terreus.埃莫丁的UDP-葡萄糖转移酶.埃莫丁葡萄糖化物合成生物学合成生物学整个细胞的催化作用.

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