在河流占主导地位的河口的不同水位下,溶解有机的利用率和组成
Xia Zhang1, Lijun Cui2, Songlin Liu3
1State Key Laboratory of Tropical Oceanography, Laboratory of Tropical Marine Bio-resources and Ecology, Guangdong Provincial Key Laboratory of Applied Marine Biology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China; Sanya National Marine Ecosystem Research Station, Tropical Marine Biological Research Station in Hainan, Chinese Academy of Sciences, and Key Laboratory of Tropical Marine Biotechnology of Hainan Province, Sanya 572000, China.
Marine pollution bulletin
|July 24, 2025
概括
微生物性酸酶 (AP) 在沿海水域的溶解有机 (DOP) 矿化中发挥着关键作用. 这项研究揭示了多样化的细菌群落及其AP活动受到环境因素和珍珠河河口的含量的影响.
科学领域:
- 环境微生物学环境微生物学
- 生物地质化学生物地质化学
- 分子生态学分子生态学
背景情况:
- 溶解有机 (DOP) 是水生生态系统中至关重要的营养素,但其生物利用性往往是有限的.
- 微生物性酸酶 (AP) 是关键的酶,可以将DOP矿化为无机酸盐,从而支持微生物生长.
- 在沿海环境中,特别是在人为压力下,AP携带微生物群落的多样性和功能仍然不太清楚.
研究的目的:
- 调查珍珠河河口 (PRE) 细菌性酸盐酶 (phoD) 和酸盐转运体 (phoD) 基因的遗传多样性和丰富性.
- 探索AP活动,微生物群落结构和环境变量 (pH,盐度,溶解无机) 之间的关系.
- 描述DOP的分子组成,并确定细菌AP的潜在基质.
主要方法:
- 使用定量PCR (qPCR) 来确定phoD和pstS基因的丰富性.
- 部分最小平方路径建模 (PLS-PM) 用于分析环境因素,微生物种群,基因丰富度和AP活动之间的关系.
- 富里埃变换离子循环子子共振质谱法 (FT-ICR MS) 用于分析DOP的分子组成.
主要成果:
- 颗粒附着部分中的AP活性主要是由环境变量 (pH,盐度) 驱动的,这些变量会影响细菌种群和phoD基因拷贝.
- 在自由生活的部分中,AP活动主要由溶解无机 (DIP) 水平控制,影响phoD社区结构和基因丰富度.
- 包括Actinobacteria,Alphaproteobacteria和Betaproteobacteria在内的各种细菌种群被确定为重要的phoD矿化剂,而picocyanobacteria则含有pstS基因. 确定CHOP化合物 (脂质) 是潜在的AP基质,占DOP总量的95%以上.
结论:
- 细菌AP在矿化特定的DOP化合物中发挥着至关重要的生态化学作用,支持河口中的浮游植物生物质.
- 这项研究强调了在珍珠河河口内的循环中,各种含有phoD的微生物的重要性.
- 来自陆地来源的DOP和DIP输入的有效管理对于保持河口生态系统健康至关重要.
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