阐明dimetomorph和difenoconazole的联合毒性:对斑马鱼不同生物过程的代际影响
Dou Wang1, Yihan Wang2, Liangang Mao3
1State Key Laboratory for Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Ecotoxicology and environmental safety
|July 24, 2025
概括
农业农药二甲型 (DMO) 和二可纳 (DFC) 在几代斑马鱼中引起氧化应激和内分泌干扰. 这些毒性影响影响父母 (F0) 和后代 (F1) 鱼,即使后代没有暴露.
科学领域:
- 环境毒理学环境毒理学
- 生态毒理学 生态毒理学
- 水生毒理学 水生毒理学
背景情况:
- 在农业中广泛应用的二甲基 (DMO) 和二可纳 (DFC) 导致它们在水生生态系统中频繁同时出现.
- 对于这些经常检测到的农药的综合跨代毒理学影响的了解有限.
研究的目的:
- 调查DMO和DFC对斑马鱼 (Danio rerio) 在多代人的联合毒理影响.
- 评估跨代的影响,包括氧化应激,亡,解毒酶活性和内分泌干扰.
主要方法:
- 急性毒性试验确定了斑马鱼胚胎中DMO和DFC的96小时LC50值.
- 根据父母 (F0) 斑马鱼暴露的LC50值来确定慢性暴露度.
- F0代斑马鱼在繁殖前暴露了60天;F1后代接受了为期7天的暴露,以评估跨代的影响.
主要成果:
- 父母 (F0) 对DMO和DFC的暴露引起了显著的氧化应激,亡,并改变了排毒酶活动 (MDA,CAT,SOD,Caspase-3,CARE,GST).
- 这些生化变化在F1代持续存在并被放大,即使持续暴露.
- 同时暴露导致内分泌干扰,以高甲状腺激素 (T3,T4) 和改变基因表达 (vtg,crh,tshb) 表示,破坏HPT轴.
结论:
- 迪梅索莫夫和二可纳在斑马鱼中产生显著的毒理作用,影响直接暴露的父母 (F0) 和他们的未暴露的后代 (F1).
- 该研究强调了这些常见的农业农药在水生生物中的跨代毒性和内分泌干扰的潜力.
- 研究结果为评估风险和保护水生生物多样性免受农药污染提供了关键的见解.
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