基于pe-ppe基因家族的Mycobacterium结核病L2.1的全球遗传多样性
Olabisi Flora Davies-Bolorunduro1, Bharkbhoom Jaemsai2, Wuthiwat Ruangchai3
1Pornchai Matangkasombut Center for Microbial Genomics, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand; Floret Center for Advanced Genomics and Bioinformatics Research, Lagos, Nigeria.
结核菌菌菌谱系2.1 (L2.1) 菌株显示有关XDR前的个人资料. 确定了包括esxR-esxS在内的pe-ppe基因中的遗传缺失,可能会影响这种结核病血统的毒性和传播.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 结核菌菌 (Mtb) 包含四个主要的血统,具有不同的地理分布.
- 东亚血统Mtb系2 (L2) 在亚洲和全球普遍存在,亚系L2.1主要存在于东南亚.
- L2.1分离物经常表现出对异化,利法和诺的耐药性,将其归类为前广泛耐药 (XDR) 菌株,构成重大公共卫生威胁.
研究的目的:
- 在L2.1菌株中研究pe-ppe基因家族内的结构变异.
- 了解L2.1 Mtb菌株中毒性和致病性的遗传基础.
- 探索已识别的遗传删除对L2.1血统的进化和传播的影响.
主要方法:
- 分析了来自8个国家的180个高质量的L2.1样本的全基因组测序 (WGS) 数据.
- 进行了比较基因组分析,以确定pe-ppe基因家族中的结构变异,特别是缺失.
- 遗传学分析用于检查特定遗传缺失的分布和聚类.
主要成果:
- 在26个L2.1基因组中确定了esxR-esxS基因段的删除,其中19个形成了主要来自越南,泰国和中国的独特类.
- 在所有检查的L2.1样本中确认了四个特定的pe-ppe基因 (wag22,ppe38,ppe50和ppe66) 的删除.
- 这些遗传变化为L2.1血统的独特特征提供了洞察力.
结论:
- 在pe-ppe基因家族中发现的遗传缺失是L2.1 Mtb血统的特征.
- 这些缺失可能在L2.1菌株的毒性,致病性和进化轨迹中发挥作用.
- 了解这些基因组变异对于解决耐药结核病的持续和传播至关重要.
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