在殖民地蓝藻细菌的粘液中检测和量化微囊
Myriam Bormans1, Océane Reignier2, Véronique Savar3
1UMR CNRS 6553 ECOBIO, University of Rennes, F-35042, Rennes, France.
概括
这项研究表明,加热和其他方法显著增加了在蓝藻细菌粘液中的微囊素 (MC) 检测,突出了细胞外聚合物质 (EPS) 在MC结合和生物可用性中的作用.
科学领域:
- 环境科学 环境科学
- 微生物学 微生物学
- 分析化学 分析化学
背景情况:
- 微囊 (MCs) 是由Microcystis物种产生的强有力的毒素.
- MCs可以从细胞中释放出来,并与粘液中的细胞外聚合物质 (EPS) 相结合.
- 准确量化MCs对于风险评估至关重要.
研究的目的:
- 调查检测和量化微囊 (MCs) 与细胞外聚合物质 (EPS) 相关的方法在蓝藻细菌粘液中.
- 为了比较通过古典过获得的MC度与涉及离心,固定和加热的增强方法.
- 评估EPS对MC总量的贡献,并了解MC变异分布.
主要方法:
- 从粘液和EPS中分离蓝藻细菌细胞.
- 使用古典过和甲醇提取方法量化MCs.
- 使用离心,冷化,固定和热溶解的增强提取.
- 对MC变体的分析.
主要成果:
- 与增强方法相比,古典过低估了颗粒物MCs.
- 加热和溶解使颗粒物MC度增加了1.6至1.9倍.
- 与附着EPS相关的MCs在总检测到的MCs中贡献了高达72%.
- 在细胞内和EPS相关的分数之间观察到MC变异的转变.
结论:
- 增强的提取方法,特别是涉及加热,对于精确的MC量化在粘液中至关重要.
- 细胞外聚合物质在结合MC中起着重要作用,影响其生物可用性.
- 需要对EPS相关的MCs被异质型细菌降解进行进一步研究.
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