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通过调节与自相关的基因,FOXO参与了抗WSSV免疫力

Xiaohan Liu¹, Ying Huang², Ziyue Zhu³

¹State Key Laboratory of Climate System Prediction and Risk Management, Nanjing University of Information Science and Technology, Nanjing, 210044, China; School of Marine Sciences, Nanjing University of Information Science and Technology, Nanjing, 210044, China; Jiangsu Province Engineering Research Center for Aquatic Animals Breeding and Green Efficient Aquacultural Technology, College of Marine Science and Engineering, Nanjing Normal University, Nanjing, 210023, Jiangsu Province, China.

Fish & shellfish immunology

|July 24, 2025

概括

叉头盒O (FOXO) 在白斑综合征病毒 (WSSV) 感染期间调节甲类动物的自基因. 这种相互作用对于天生的免疫力至关重要,因为抑制FOXO会降低对WSSV的生存率.

科学领域:

免疫学免疫学免疫学
分子生物学分子生物学
甲动物生物学甲动物生物学

背景情况:

叉头盒O (FOXO) 蛋白质是已知的自的调节者,通过与自相关 (ATG) 基因的相互作用.
鱼在甲动物免疫力中的FOXO作用,特别是关于像白斑综合征病毒 (WSSV) 这样的病毒感染,仍然在很大程度上未被探索.

研究的目的:

研究FOXO的作用及其对ATG基因的调节在 *Eriocheir sinensis*对WSSV的先天免疫反应中的作用.
为了确定FOXO是否影响WSSV感染进展和宿主存活.

主要方法:

在*Eriocheir sinensis*中鉴定和对七个ATG基因进行了基因分析.
在各种组织和WSSV刺激后分析EsFOXO和ATG基因表达.
通过RNA干扰 (RNAi) 来降低EsFOXO表达,并评估其对ATG基因表达,VP28水平和WSSV感染的生存率的影响.

主要成果:

在 *E. sinensis* 中发现了七个ATG基因,显示出与其他甲类同类动物的进化接近.
埃斯福克索和已识别的ATG基因在多种组织中被表达,并在WSSV感染时被上调.
击败EsFOXO导致改变ATG基因表达,增加VP28水平,并显著降低了WSSV感染的的生存率.

结论:

福克索在甲动物对WSSV的天生的免疫反应中发挥着重要作用.
FOXO调节ATG基因,这表明一种涉及自的机制在抗病毒防御.
准FOXO可能是管理甲类动物中WSSV爆发的潜在策略.

关键词:

自自是一种自的过程.这里是Eriocheir sinensis的原点.福克斯OFOXO 在线播放白色斑点综合征病毒病毒.

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