相关实验视频

Updated: Sep 14, 2025

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Production of Pseudotyped Particles to Study Highly Pathogenic Coronaviruses in a Biosafety Level 2 Setting

Published on: March 1, 2019

HKU5蝙蝠merbecoviruses与蝙蝠和子ACE2作为进入受体进行接触

Mia Madel Alfajaro^1,2, Emma L Keeler^1,3, Ning Li⁴

¹Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT, USA.

Nature communications

|July 24, 2025

概括

蝙蝠冠状病毒HKU5使用Pipistrellus abramus蝙蝠ACE2,而不是人类ACE2,进入细胞. 这一发现影响了对病毒传播的理解,并为广泛的冠状病毒疫苗的开发提供了信息.

科学领域:

病毒学病毒学
结构生物学结构生物学
传染性疾病传染性疾病

背景情况:

识别病毒受体对于疫情准备至关重要.
中东呼吸综合征冠状病毒 (MERS-CoV) 使用DPP4,但相关蝙蝠β冠状病毒的受体通常是未知的.
蝙蝠merbecovirus HKU5在人类细胞中表现出进入限制.

研究的目的:

为了识别蝙蝠merbecovirus HKU5.5使用的细胞受体.
描述HKU5尖端蛋白及其受体之间的相互作用.
评估跨物种传播的可能性,并为疫苗开发提供信息.

主要方法:

使用伪型和全长的HKU5病毒进行入口测试.
进行了冷电子显微镜来分析病毒受体复合体.
进行结构引导的突变发生,以研究尖端受体相互作用.

主要成果:

HKU5使用Pipistrellus abramus蝙蝠ACE2,但不是人类ACE2或DPP4作为其细胞受体.
尖峰-ACE2相互作用机制不同于已知的其他利用ACE2的冠状病毒.
在MERS-CoV疫苗血清中,HKU5.5的中和能力较差.
此外,HKU5还可以使用美国子和牛ACE2,这表明类动物是潜在的宿主.

结论:

蝙蝠merbecovirus HKU5采用一个独特的ACE2-依赖的入口途径.
这些发现揭示了类动物作为merbecoviruses的潜在中间宿主.
了解受体使用对于监测和开发泛美尔贝病毒对策至关重要.

更多相关视频

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Stability and Structure of Bat Major Histocompatibility Complex Class I with Heterologous β2-Microglobulin

Published on: March 10, 2021

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Use of Viral Entry Assays and Molecular Docking Analysis for the Identification of Antiviral Candidates against Coxsackievirus A16

Published on: July 15, 2019

相关概念视频

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

Receptor-mediated Endocytosis

Receptor-mediated Endocytosis

Receptor-mediated endocytosis is when bulk amounts of specific molecules are imported into a cell after binding to cell surface receptors. The molecules bound to these receptors are taken into the cell through inward folding of the cell surface membrane, which is eventually pinched off into a vesicle within the cell. Structural proteins, such as clathrin, coat the budding vesicle.
Clathrin-Mediated Endocytosis of LDL
One well-characterized example of receptor-mediated endocytosis is the...

Retrovirus Life Cycles

Retrovirus Life Cycles

Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...