使用 Actinobacillus succinogenes 通过半同步的糖化和发酵,从林氏纤维素生物质中增强酸生产
Dwini Normayulisa Putri1, Meka Saima Perdani2, Ibnu Maulana Hidayatullah3,4
1Research Center for Biomass and Bioproducts, National Research and Innovation Agency, Cibinong, Bogor, 16911, Indonesia. dwin014@brin.go.id.
Bioprocess and biosystems engineering
|July 24, 2025
概括
半同时的糖化和发酵 (SSSF) 显著增加了从林氏纤维素生物质中产生酸 (SA) 的产量. 这种新的方法可以提高21-26%的SA产量,而不是同时糖化和发酵 (SSF).
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
- 微生物生产 微生物生产
背景情况:
- 纤维素生物质是生产有价值化学品的可持续原料.
- 酸 (SA) 是一种关键的平台化学物质,具有多种工业应用.
- 优化发酵过程对于高效的生物质SA生产至关重要.
研究的目的:
- 评估半同时糖化和发酵 (SSSF) 对于增强酸生产的有效性.
- 为了比较SA产量从使用SSSF与同时糖化和发酵 (SSF) 的纤维纤维素.
- 为了研究过程参数对Actinobacillus succinogenes的SA生产的影响.
主要方法:
- 纤维素生物质的预处理 (棕油的空果束和甘包).
- 应用一个6小时的预水解步骤,然后进行一个48小时的SSSF过程.
- 在不同的条件下种植Actinobacillus succinogenes (注射液度,基质类型,基质度).
主要成果:
- SSSF从棕油空果束 (OPEFB) 获得了1.18g/L的SA,从甘包 (SB) 获得了1.19g/L.
- 与SSF相比,SSF显示SA度增加了21-26%,而SSF则显示SA度增加了21-26%.
- 无菌和基质度显著影响了OPEFB的SA产量,但不是从SB.
结论:
- 该SSSF配置显著增强了从林氏纤维素生物质中酸的生产.
- 与传统的SSF工艺相比,SSF提供了一种更有效的SA生物合成方法.
- 进一步优化SSSF参数可以导致从可持续原料中获得更高的SA产量.
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