利莫西拉ctobacillus reuteri-Fusobacterium nucleatum 相互作用 调节生物膜组成和免疫性
Luming Yang1, Gopu Sriram1, Ren Jie Jacob Chew1
1Faculty of Dentistry, National University of Singapore, Singapore.
Journal of periodontal research
|July 25, 2025
概括
与Fusobacterium nucleatum的Limosilactobacillus reuteri联合聚合显著减少了生物膜中的F. nucleatum,并降低了其免疫性. 这种相互作用也会降低 Porphyromonas gingivalis,影响口腔微生物群落.
科学领域:
- 口腔微生物学 口腔微生物学
- 益生菌 益生菌 益生菌
- 生物膜的形成过程.
背景情况:
- 目前尚不清楚Limosilactobacillus reuteri和口腔细菌之间的相互作用.
- Fusobacterium nucleatum 在口腔生物膜发育中起着关键作用.
- L. reuteri是一种已知的益生菌,具有潜在的口腔健康益处.
研究的目的:
- 描述L. reuteri和F. nucleatum之间的联合聚合.
- 确定这种相互作用对生物膜组成的影响.
- 评估口服细菌聚合物的免疫性影响.
主要方法:
- 在体外实验中使用L. reuteri菌株 (DSM 17938,ATCC PTA 5289) 和口腔细菌 (F. nucleatum ATCC 25586,P. gingivalis W50) 的实验.
- 管内凝聚试验和共聚焦显微镜来评估应变相互作用.
- 通过显微镜和培养进行生物膜组成分析.
- 使用人类细胞系 (纤维细胞,上皮细胞,单细胞) 进行免疫性评估.
主要成果:
- 这两种L. reuteri菌株与F. nucleatum联合聚合,使生物膜中的F. nucleatum减少了1000倍.
- 凝聚显著降低了F. nucleatum. 的免疫性.
- 此外,L. reuteri的凝聚还导致生物膜内的P. gingivalis减少了50%.
结论:
- L. reuteri通过与F. nucleatum. 联合聚合,表现出新的益生菌机制.
- 这种相互作用调节了F. nucleatum的免疫性及其作为桥梁物种的作用.
- 由L. reuteri凝聚物改变的生物膜组成限制了口腔失生症.
相关概念视频
01:29Biofilms
278
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
278
01:28Gene Regulation in Microbial Communities: Quorum Sensing
93
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
93