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在Corynebacterium glutamicum中与葡萄糖进行工程酶代谢和结合,以制造高效的微生物细胞工厂

Nana Ding¹, Jianan Yan¹, Qian Luo¹

¹National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou 311300, China.

Journal of agricultural and food chemistry

|July 25, 2025

概括

这项研究对Corynebacterium glutamicum进行了改造,使其能够更好地代谢西洛斯,提高其使用来自林氏纤维素生物质的混合糖用于生物基化学品生产的能力.

科学领域:

生物技术是生物技术.
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

作为一个关键的基纤维素生物质成分,西洛由于运输和代谢瓶,在微生物中面临着低效的利用.
优化纤维素同化对于可持续的生物基化学品从可再生资源生产至关重要.

研究的目的:

为了增强西洛斯的新陈代谢和在Corynebacterium glutamicum中共同利用葡萄糖和西洛斯.
设计新陈代谢和转录途径,以改善西洛斯的同化.

主要方法:

通过异质表达西洛斯异构酶 (XylA) 和西洛斯酶 (XylB) 构建了西洛斯同化途径.
鉴定并引入了来自大肠杆菌的西洛斯输送物XylE,以改善西洛斯的吸收.
采用适应性实验室进化来选择增强的西洛斯利用.

主要成果:

改造后的C. glutamicum菌株显示出改善了西洛斯的代谢能力.
XylA和XylB的异质表达增强了西洛斯的同化.
引入XylE改善了氧化糖的运输和减轻了葡萄糖的抑制.
选择的菌株WTABE_ALE6证明了高效的葡萄糖和西洛斯的联合利用.

结论:

代谢工程和转录调节策略显著增强了C. glutamicum. 中的西洛斯利用率.
优化纤维素运输和同化通路是高效混合糖发酵的关键.
这项工作有助于为生物基化学品生产转化纤维纤维化生物质.

关键词:

在Corynebacterium glutamicum的研究中,我们发现葡萄糖和西洛糖的结合.适应性实验室进化的演变.氧化糖的新陈代谢

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