在4°C和23°C-25°C的储存对Dunkin Hartley海豚的全血计数的影响
Yan-Ni Huang1, Yu-Han Xie2,3, Jian-An Ling1
1Department of Occupational Health and Toxicity Assessment, Guangxi Zhuang Autonomous Region Institute for the Prevention and Treatment of Occupational Disease, Nanning, China.
Veterinary clinical pathology
|July 25, 2025
概括
在4°C的温度下储存Dunkin Hartley几内亚猪的血液样本,可保持全血细胞计 (CBC) 的稳定性长达24小时. 建议在室温以上4°C的冷藏,以延迟对这些动物进行CBC测试.
科学领域:
- 兽医血液学 兽医血液学
- 实验室动物科学 动物科学
- 比较病理学比较病理学
背景情况:
- 敦金·哈特利 (Dunkin Hartley) 试验猪在研究中被广泛使用.
- 有限的数据存在于各种存储条件下他们的血液样本的稳定性.
- 了解样本稳定性对于可靠的血液学分析至关重要.
研究的目的:
- 评估储存温度和时间对Dunkin Hartley试验猪的全血细胞计 (CBC) 参数的影响.
- 为了确定最佳的储存条件,以保持血液样本的完整性.
- 为标准化实验室协议提供数据.
主要方法:
- 采集了20只敦金哈特利 (Dunkin Hartley) 试验猪的血液样本 (EDTA-K2管).
- 在4°C和23°C-25°C保存样品.
- 使用Hemavet 950FS分析仪在多个时间点进行CBC分析,长达120小时.
- 利用三向重复测量ANOVA分析时间,温度和性别的影响.
主要成果:
- 白细胞 (WBC),异质细胞,淋巴细胞,单细胞和红细胞 (RBC) 在4°C保持稳定.
- 白血细胞,单细胞和红血细胞在23°C-25°C时稳定.
- 在两种温度下长时间储存后,血小板 (PLT) 计数显著增加,特别是在72-96小时后.
结论:
- 为了保持CBC的稳定性,最好在4°C的温度下储存Dunkin Hartley几内亚猪血液样本.
- 对于延迟的CBC检测,样品储存时间不应超过24小时,以确保可靠的结果.
- 这些发现为研究几内亚猪的样本处理提供了最佳实践信息.
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