该miR156u/v-MhDIV3模块通过增强MhNRAMP1表达在Malus hupehensis中调节的吸收和损伤
Jianfei Song1, Junhong Yan1, Baozhen Sun1
1College of Horticulture Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.
Plant, cell & environment
|July 25, 2025
概括
果树中的吸收受到DIVARICATA (DIV) 转录因子 (TF) MhDIV3的积极调节,这增加了压力损伤. 抑制MhDIV3或过度表达miR156可以减少的积累和毒性.
科学领域:
- 植物生物学 植物生物学
- 环境毒理学环境毒理学
- 分子遗传学 分子遗传学
背景情况:
- (Cd) 是一种有毒的重金属,影响作物生产和人类健康.
- 在Malus hupehensis中,Cd的吸收是由MhNRAMP1介导的,但DIVARICATA (DIV) 转录因子的作用尚不清楚.
研究的目的:
- 为了研究DIV转录因子在Malus hupehensis中的应激反应中的功能.
- 阐明Cd吸收和应力耐受性的调节机制,涉及DIV及其上游监管机构.
主要方法:
- 从Malus hupehensis中分离了九个DIV基因.
- 在Cd压力下进行基因表达分析.
- 在M. hupehensis根,果和番茄植物中,基因沉默和过度表达.
- 对反应性氧物种 (ROS),马隆迪化物 (MDA) 和Cd2+吸收的分析.
- 路西法酶记者测定证实TF结合和miRNA调节.
主要成果:
- MhDIV3,一个R2R3-MYBTF,对Cd.表现出最强的反应.
- 抑制MhDIV3可以减少Cd诱导的氧化损伤和Cd2+吸收.
- 过度表达MhDIV3通过增加Cd2+吸收加剧了Cd压力.
- 在Cd压力下,MhDIV3积极调节MhNRAMP1的表达.
- miR156u/v作为MhDIV3的上游负调节剂,减少Cd吸收和应激损伤.
结论:
- 在miR156u/v-MhDIV3模块积极调节Cd吸收和压力损伤在Malus hupehensis.
- 通过上调MhNRAMP1的表达,MhDIV3可以提高Cd的耐受性.
- 通过miR156u/v介导的MhDIV3抑制提供了减轻作物中Cd污染的潜在策略.
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