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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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提高智能手机光显微镜设备的生物标志物检测和成像性能.

Muhammad Ahsan Sami1, Muhammad Nabeel Tahir1, Umer Hassan1,2

  • 1Department of Electrical and Computer Engineering, School of Engineering, Rutgers, The State University of New Jersey, New Brunswick, NJ 08901, USA.

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概括

智能手机光显微镜 (SFM) 可以通过计算噪声校正来改进. 3D平均和高斯过器显著提高粒子检测和白细胞成像的图像质量,改善诊断潜力.

关键词:
图像质量提升 图像质量提升影像过器 影像过器血清细胞是白细胞.微型/纳米颗粒的使用.智能手机的光显微镜 (SFM)

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科学领域:

  • 生物医学光学 生物医学光学
  • 计算成像技术的成像
  • 显微镜的使用方法

背景情况:

  • 智能手机配合的3D光显微镜 (SFM) 是诊断和生物制造中宝贵的工具.
  • 之前的工作证明了SFM在微纳米粒子传感和白细胞成像中的实用性.
  • 提高SFM成像性能和降低复杂性是关键的研究目标.

研究的目的:

  • 为SFM开发和评估一种新的计算噪声校正应用程序.
  • 为了提高SFM的成像性能和操作简单性.
  • 评估3D平均和高斯过器在SFM图像中的降噪效果.

主要方法:

  • 采用了3D平均和3D高斯过器,具有不同的内核大小和标准偏差来纠正噪声.
  • 使用定制设计的SFM.使用成像光珠 (0.88.3μm).
  • 通过自定义算法 (AQAFI) 使用信号差异与噪声比率 (SDNR) 和对比度与噪声比率 (CNR) 的量化图像质量改进.
  • 将最佳过器应用于被光标记的人类外周血液白细胞的图像.

主要成果:

  • 计算过器显著提高了粒子检测的信号质量.
  • 使用21 × 21 × 21内核实现了最优的性能,用于平均过器.
  • 对于高斯过器,最优的结果是 σ=5 和 21 × 21 × 21 内核.
  • 噪音校正改善了在各种条件下捕获的白细胞图像的清晰度.

结论:

  • 使用3D平均和高斯过器进行计算噪声校正,有效地增强了SFM成像.
  • 开发的应用程序简化了SFM操作,并提高了图像质量.
  • 这些过器广泛适用于各种光显微镜设计,增强其功能.