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将光学分辨率推向几纳米尺度,通过dSTORM对扩展样本-凝复合材料的成像

Jimmy Ching-Cheng Hsu^1,2, T Tony Yang^1,2

¹Department of Electrical Engineering, National Taiwan University, Taipei 10617, Taiwan.

Gels (Basel, Switzerland)

|July 25, 2025

概括

标记后的十倍强壮扩展显微镜 (plTREx) 与直接随机光学重建显微镜 (dSTORM) 结合,实现了低于10nm的超结构分辨率. 这种plTREx-dSTORM方法增强了成像稳定性和信号密度,用于细胞蛋白质可视化.

科学领域:

生物物理学的生物物理.
显微镜的使用方法
细胞生物学细胞生物学

背景情况:

直接随机光学重建显微镜 (dSTORM) 提供纳米级分辨率 (10-20 nm),但难以获得超结构细节.
扩展显微镜 (ExM) 与dSTORM相结合可以达到10nm以下的分辨率,但受到扩展因子和高折扩展的技术挑战的限制.

研究的目的:

开发一个强大且兼容的工作流程,将高折扩展显微镜与dSTORM集成用于超结构成像.
为了克服成像技术的局限性,高度扩展的标本用于增强分辨率.

主要方法:

推出了标签后十倍强大的扩展显微镜 (plTREx) 以提高水凝机械稳定性和光信号密度.
优化了重新嵌入协议,以将plTREx与dSTORM集成,防止凝收缩.
开发了一个名为plTREx-dSTORM的工作流程,用于兼容的广场和dSTORM成像.

主要成果:

实现了低于10nm的成像分辨率,使细胞蛋白的超结构解释成为可能.
证明了扩张水凝的增强机械稳定性.
改善了宽场和dSTORM成像平台的光信号密度.

结论:

plTREx-dSTORM有效地弥合了电子显微镜和光学显微镜之间的分辨率差距.
这种综合方法为细胞超结构提供了高度精细的成像能力.
开发的工作流程有助于对生物标本进行先进的纳米尺度成像.

关键词:

SMLMLM SMLMLM 这是一个很大的问题.dSTORM 这是一个风暴.膨胀显微镜 (ExM) 的使用超分辨率显微镜的显微镜.

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