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评估铜氧化物纳米材料对肠道相关细菌的抗菌特性:一个多方面的方法

Tia A Wardlaw¹, Abdulkader Masri¹, David M Brown¹

¹NanoSafety Research Group, School of Engineering and Physical Sciences, Heriot-Watt University, Edinburgh EH14 4AS, UK.

Nanomaterials (Basel, Switzerland)

|July 25, 2025

概括

氧化铜纳米材料 (CuO NMs) 对肠道细菌如大肠杆菌表现出抗菌作用. O NMs降低了细菌的生存能力,可能是通过反应性氧物种 (ROS) 生产,突出了对强大的测试方法的需求.

科学领域:

环境科学环境科学
材料科学材料科学材料科学
微生物学微生物学

背景情况:

抗菌纳米材料 (NM) 的使用越来越多,引发了人们对人体摄入和肠道微生物群失生症的担忧.
研究主要集中在对病原体的NM毒性上,忽视了对有益的开始性肠道细菌的影响.

研究的目的:

为了研究氧化铜 (CuO) NMs对肠道相关细菌的体外抗菌活性: Escherichia coli K12,Enterococcus faecalis 和 Lactobacillus casei.
评估反应性氧物种 (ROS) 生产作为CuO NM毒性的机制的作用.

主要方法:

评估CuO NM对细菌生长和活力的影响 (100-300μg/mL),使用板数,光密度 (OD) 测量,扩散分析和活/死光成像.
在NM暴露后通过DCFH-DA光成像进行量化ROS产生.

主要成果:

在大多数测试中,CuO NMs以度依赖的方式降低了细菌活力,OD测量和板数是最敏感的.
埃舍里希亚大肠杆菌K12对CuO NM毒性表现出最高的敏感性.
在所有测试的细菌菌株中,CuO NM暴露诱导了ROS的产生.

结论:

CuO NMs对关键的肠道共生细菌表现出抗菌活性.
通过NM介导的ROS产生可能是导致细菌活力降低的因素.
测试的组合,特别是盘子数和OD测量,提供了对摄入NMS的抗菌性质的可靠评估.

关键词:

它是一种抗菌药物,具有抗菌作用.铜氧化物铜氧化物在体外 (in vitro) 的情况下.纳米材料的使用方法纳米毒理学的纳米毒理学有活性氧物种的反应性氧物种.

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