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相关概念视频

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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相关实验视频

Updated: Sep 13, 2025

Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method
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Measuring Nitrite and Nitrate, Metabolites in the Nitric Oxide Pathway, in Biological Materials using the Chemiluminescence Method

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用可见光驱动的酸盐与3,3',5,5'-四甲基化的直接色度检测.

Xianming Li1, Ling Li1, Heye Lv2

  • 1Analytical & Testing Center, Sichuan University, Chengdu 610064, China.

Analytical chemistry
|July 25, 2025
PubMed
概括

这项研究表明,酸盐与3,3反应.

科学领域:

  • 分析化学 分析化学
  • 生物化学 生物化学
  • 频谱学是一种光谱学.

背景情况:

  • 3,3',5,5'-四甲丁 (TMB) 在色度酸盐测定中使用.
  • 现有的基于TMB的酸盐测试及其机制存在争议.
  • 经典格里斯测定是检测亚酸盐的标准.

研究的目的:

  • 为了阐明TMB和酸盐之间的反应机制.
  • 为了识别TMB-化物反应的最终产物.
  • 开发一种改进的基于TMB的色度酸盐测定方法.

主要方法:

  • 电子喷雾电离质谱仪 (ESI-MS) 用于反应产品的识别.
  • 谱光测量用于色度检测.
  • 蓝色LED辐射以增强反应动力学.

主要成果:

  • 亚酸盐通过二化与TMB反应,形成黄色的二-TMB,而不是TMB2+.
  • 可见光促使diazo-TMB进入激发状态,从而通过光还原或光敏感氧化形成TMB+•.
  • 一个光驱测试实现了0.1μM的酸盐检测极限.

结论:

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Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
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Detection of 3-Nitrotyrosine in Atmospheric Environments via a High-performance Liquid Chromatography-electrochemical Detector System
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  • 澄清了TMB与酸盐的反应机制,其中包括二化和光诱导氧化.
  • 开发了一种新的,灵敏的,光增强的色度测试方法来检测化物.
  • 该测试显示了在尿样中检测酸盐和诊断尿路感染的潜力.