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使用蛋白质保留扩展显微镜可视化培养细胞中超细核结构的协议

Takaaki Okamoto¹, Masahide Fukada², Akio Masuda²

¹Department of Cellular Pathology, Institute for Developmental Research, Aichi Developmental Disability Center, Kasugai, Aichi 480-0392, Japan; Department of Orthopaedic Surgery, Aichi Developmental Disability Center Central Hospital, Kasugai, Aichi 480-0392, Japan.

|July 25, 2025

概括

这项研究引入了蛋白质保留扩展显微镜 (proExM),以可视化细胞中的核蛋白分布. 这种具有成本效益的协议揭示了用于研究转录和RNA处理的超细核组织.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
显微镜的使用方法

背景情况:

核中包含复杂的功能网络,包括DNA,RNA和蛋白质.
这些网络对于核代谢至关重要,包括转录和RNA处理.
可视化亚核蛋白分布是理解这些过程的关键.

研究的目的:

通过蛋白质保留扩展显微镜 (proExM) 呈现可视化亚核蛋白质分布的协议.
为了研究培养细胞中的超细核组织.

主要方法:

培养细胞中核蛋白的免疫覆盖.
使用proExM技术进行样本扩展.
将扩展样品粘合到玻璃盖片上.
使用共聚焦显微镜对扩展样品进行成像.

主要成果:

该协议允许可视化亚核蛋白分布.
它使得研究超细核组织成为可能.
该方法是可重复的和具有成本效益的.

结论:

蛋白质保留膨胀显微镜 (proExM) 是研究核组织的一个有价值的工具.
该协议为详细的核结构分析提供了一种可复制和成本效益的方法.

关键词:

细胞生物学细胞生物学细胞培养培养的细胞培养.显微镜的使用方法

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