利文PRO-CR通过TPH1调解5-HT和肠道微生物群,以改善缓慢过渡性便秘
Fei Xia1, Qibin He1, Shenglin Wang1
1Department of Gastroenterology, The Affiliated Jiangning Hospital of Nanjing Medical University, Nanjing, Jiangsu 211100, China.
Molecular immunology
|July 25, 2025
概括
这项研究表明,Leven PRO-CR是一种益生菌混合物,通过改善肠道微生物群平衡和增强肠道运动性,有效治疗慢过渡性便秘 (STC). 它恢复了结肠健康和功能,为STC患者提供了一种新的治疗方法.
科学领域:
- 胃肠病学 胃肠病学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 慢过渡性便秘 (STC) 是一种慢性功能性胃肠道疾病,其特点是长时间的结肠过渡时间和显著的患者不适.
- 目前对STC的治疗选择有局限性,需要探索针对肠道运动和微生物群的新型治疗方法.
- 肠道微生物群在调节肠道功能的过程中起着至关重要的作用,肠道功能失调与STC的病理生理学有关.
研究的目的:
- 研究一种新型益生菌混合物Leven PRO-CR在缓解慢过渡便秘症状方面的治疗效果.
- 阐明Leven PRO-CR调节肠道微生物群组成并增强肠道运动的基本机制.
- 评估Leven PRO-CR对结肠组织完整性,细胞亡和关键神经化学标志物的表达的影响,这些标志物与肠道功能有关.
主要方法:
- 为实验评估建立了洛佩拉胺诱导的STC小鼠模型.
- 评估胃肠道过境参数,包括排便频率,便含水量和肠道过境速率.
- 组织病理学检查 (H&E,IHC,TUNEL),结肠组织的分子分析 (qPCR,西式涂抹,免疫光),以及16S rDNA测序用于肠道微生物群的概况.
主要成果:
- 利文PRO-CR显著改善了STC症状,减少了第一次排便时间,增加了便频率,便含水量和肠道过渡率.
- 使用Leven PRO-CR的治疗恢复了结肠组织完整性,通过c-Kit/SCF信号传递增强了Cajal (ICCs) 间歇细胞的存活率,并减少了亡.
- 利文PRO-CR通过上调TPH1和增加5-HT水平来调节血清素 (5-HT) 途径,同时也影响肠道神经标记物 (NOS1,BDNF,TRPV1,GDNF) 并恢复肠道微生物多样性和平衡.
结论:
- 利文PRO-CR在缓解慢过渡性便秘方面显示出显著的治疗潜力.
- 利文PRO-CR的有益作用归因于其恢复肠道微生物群平衡的能力,通过5-HT通路调节增强肠道运动性,并改善结肠组织完整性.
- 莱文PRO-CR代表了一种有前途的新型治疗剂,通过解决该疾病的多种病理生理方面的方法来管理缓慢过渡性便秘.
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