在不同的农业废物发酵床中,耐药基因和碳水化合物酶基因的动态变化,基于元基因组学分析
Jinxia Fan1, Yiping Guo1, Hao Yang1
1College of Engineering, Northeast Agricultural University, Harbin 150030, PR China.
Ecotoxicology and environmental safety
|July 25, 2025
概括
选择特定的发酵床材料可以减少猪场废物中的抗生素耐药基因 (ARG). 高素含量降低了ARG,而纤维素和半纤维素增加了它们,为环境污染控制提供了见解.
科学领域:
- 环境微生物学 环境微生物学
- 农业科学 农业科学
- 分子生物学分子生物学
背景情况:
- 养猪场废弃物发酵床是抗生素耐药性基因 (ARG) 的主要来源.
- 来自这些床的ARG可以污染土壤生态系统.
- 了解不同床材中的ARG动态对于缓解至关重要.
研究的目的:
- 研究各种发酵床材料对ARG丰度和分布的影响.
- 为了阐明控制ARG积累的机制,以响应床组成.
- 为选择有效的材料提供基础,以减少ARG污染.
主要方法:
- 建立了四种不同的发酵床处理方法:米 (RH),米茎 (RS),玉米茎 (CS) 和棉花茎 (CTS) 用粉.
- 使用元基因组测序来分析ARG的动态变化和分布.
- 在床材成分和ARG相关的酶活动之间进行了相关性分析.
主要成果:
- 甘酸酸酶与增加的ARG相关,而甘酸转移酶与减少的ARG相关.
- 宁促进了甘酸酶的积累,而纤维素和半纤维素促进了甘酸转移酶的积累.
- 高素含量降低了ARG积累,而高纤维素和半纤维素含量增加了它. 发现特定的细菌属 (例如,伪omonas) 在它们的细胞膜上存在ARG.
结论:
- 发酵床材料的组成显著影响ARG的丰度.
- 富含素的材料有效地减少了ARG的积累.
- 这些发现支持选择合适的床材,以减轻从畜牧业经营中传播到环境的ARG.
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