在受热应力影响的爬行草叶子中进行蛋白质分解活动
Qianqian Fan1, David Jespersen1
1Dep. of Crop and Soil Sciences, University of Georgia, Griffin Campus, 1109 Experiment St., Griffin, GA 30223, United States.
概括
热应激通过蛋白酶和爬行曲草中的无素-蛋白酶系统 (UPS) 增强了蛋白质降解. 耐热线表现出较低的活性,保持较高的蛋白质含量,以提高耐热性.
科学领域:
- 植物生理学 植物生理学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 通过蛋白酶和无素-蛋白酶系统 (UPS) 增强蛋白质降解对于植物热应激反应至关重要.
- 有限的研究存在于热应激下草的同时蛋白酶和UPS活动分析.
- 爬行曲草 (Agrostis stolonifera L.) 是一个有价值的凉季草草,需要耐热.
研究的目的:
- 在热应激下,量化不同爬行曲草系的蛋白酶和UPS活动.
- 为了检测已知的蛋白酶和UPS基因对热的反应表达的变化.
- 阐明蛋白质降解在爬行草的耐热机制中的作用.
主要方法:
- 选择耐热和敏感的爬行草线.
- 暴露植物进行控制 (20/15°C) 和热应激 (38/33°C) 35天.
- 测量了蛋白酶和UPS活动,并分析了基因表达水平.
主要成果:
- 在热应激下,蛋白质降解显著增加,由蛋白酶和UPS活动的增加表明.
- 一个耐热系 (S11 729-10) 呈现较低的蛋白酶和UPS活动,与较高的蛋白质含量和耐热性相关.
- 基因表达在线之间有所不同;热敏的克伦肖随着热应激的进展而表达的减少.
结论:
- 这项研究首次同时分析了多年草中对热应激反应的蛋白酶和UPS活性.
- 降低蛋白酶和UPS活动有助于增强爬行草的耐热性.
- 了解这些蛋白质降解途径为改善草草的耐热性提供了洞察力.
相关概念视频
Responses to Heat and Cold Stress
13.9K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.9K
Adaptations that Reduce Water Loss
26.3K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
26.3K
Regulation of Transpiration by Stomata
29.1K
During photosynthesis, plants acquire the necessary carbon dioxide and release the produced oxygen back into the atmosphere. Openings in the epidermis of plant leaves is the site of this exchange of gasses. A single opening is called a stoma—derived from the Greek word for “mouth.” Stomata open and close in response to a variety of environmental cues.
29.1K
Responses to Salt Stress
13.4K
Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
13.4K
Thermal Stress
2.6K
If the temperature of an object is changed while it is prevented from expanding or contracting, the object is subjected to stress. The stress is compressive if the object expands in the absence of constraint and tensile if it contracts. This stress resulting from temperature change is known as thermal stress. It can be quite large and can cause damage. To avoid this stress, engineers may design components so they can expand and contract freely. For instance, on highways, gaps are deliberately...
2.6K
Other Stress Responses in Bacteria
67
Bacteria have global regulatory systems that control several types of stress mechanisms. These include Pho regulon and the heat shock response, which are essential systems for environmental adaptation, such as nutrient limitation and proteotoxic stress. The Pho regulon and the heat shock response exemplify bacterial resilience, enabling rapid adaptation to fluctuating environmental conditions.Pho RegulonBacteria require phosphorus for essential cellular processes, including nucleic acid...
67


