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设计一种具有增强生理稳定的单链血管内皮生长因子,以加速伤口愈合

Ying-Wen Wang¹, Tanysha Chi-Ying Chen¹, Ching-Wei Luo²

¹Department of Life Sciences and Institute of Genome Sciences, National Yang Ming Chiao Tung University, Taipei 112, Taiwan.

International journal of biological macromolecules

|July 25, 2025

概括

将血管内皮生长因子 (VEGF) 改造为单链结构 (V165-CTP-V165) 以提高稳定性和半衰期. 这种增强的VEGF通过促进血管形成和组织修复来加速伤口愈合.

科学领域:

生物材料科学生物材料科学
分子生物学分子生物学
再生医学是一种再生医学.

背景情况:

血管内皮生长因子 (VEGF) 对于血管生成至关重要,但由于不稳定性和短半衰期而面临临临床限制.
目前的VEGF疗法受到维护二元稳定性和实现持续生理存在的挑战的阻碍.

研究的目的:

设计一种新的单链VEGF结构 (V165-CTP-V165) 以提高稳定性和延长生物活性.
评估V165-CTP-V165在促进伤口愈合和组织再生方面的治疗潜力.

主要方法:

将VEGF-A165转化为单链结构 (V165-CTP-V165),使用人类胆管性腺激素的C终端 (CTP) 作为链接器.
评估了体外 (内皮细胞增殖,迁移,管形成) 和体内 (新血管化,伤口关闭) 的生物活性.
测量了药理动力学参数,包括血液和伤口排泄物中的半衰期.

主要成果:

在体外,V165-CTP-V165的生物活性与原生VEGF-A165 (rhVEGF) 的生物活性相当.
CTP修改显著增加了VEGF的半衰期,在血液中增加了4.5倍,在伤口排泄物中增加了6倍.
在小鼠模型中,单次局部应用V165-CTP-V165加速了伤口关闭,增强了新血管和组织修复.

结论:

V165-CTP-V165是一种有前途的基于VEGF的治疗生物材料,具有更好的稳定性和延长的生理疗效.
这种工程 VEGF 构造具有在伤口护理和组织再生中的应用的巨大潜力.
在临床环境中,CTP链接器战略提供了一种可行的方法,以克服本地VEGF在临床环境中的局限性.

关键词:

新血管化的新血管化.在VEGF中,VEGF是VEGF.伤口愈合伤口愈合

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