维他费林A和维他诺利德A介导的银纳米颗粒准细菌的运动性,新陈代谢和对大肠杆菌的应激适应

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Moumita Sil¹, Anamitra Goswami², Dipro Mukherjee³

¹Biological Sciences Division, Indian Statistical Institute, 203. B.T Road, 700108 Kolkata, India.

Nanomedicine : nanotechnology, biology, and medicine

|July 25, 2025

概括

来自Withania somnifera的生物性银纳米颗粒显示出对大肠杆菌的强烈抗菌作用,毒性低. 这些植物性纳米颗粒为传统抗微生物药物提供了一个有希望的替代品.

科学领域:

纳米技术纳米技术
微生物学微生物学
药理学药理学是指药理学的学科.

背景情况:

银纳米粒子 (AgNPs) 以其抗微生物特性而闻名.
植物介导合成为纳米粒子生产提供了一种绿色和可持续的方法.
维坦尼亚 (Withhania somnifera) 是一种具有已知的生物活性化合物的药用植物.

研究的目的:

使用Withania somnifera根提取物合成和表征生物原银纳米粒子 (Bio-AgNPs).
为了评估Bio-AgNPs对格拉姆阴性细菌的抗菌疗效.
评估生物AgNPs的生物安全性和细胞毒性.

主要方法:

使用Withania somnifera根提取物合成生物AgNP.
使用UV-Vis光谱,TEM,DLS和泽塔电位测量的表征.
通过最小抑制度 (MIC) 测试评估的抗菌活性.
使用RNA测序 (RNA-seq) 调查的作用机制.
在人类细胞系上评估的细胞毒性 (WI-38,HEK-293).

主要成果:

生物AgNP表现出特有的UV-Vis峰值,晶体核心,水力动力学尺寸和负泽塔潜力.
对大肠杆菌K12具有有效的抗菌活性,超过了AgNO3.
RNA-seq揭示了细菌运动和营养吸收受损,以及氧化应激反应.
高细胞活力 (>80%) 在细胞毒性测定中观察到高达156.25ppm的细胞活力.

结论:

维坦尼亚·索姆尼费拉衍生的生物AgNP对格拉姆阴性细菌有效.
这些纳米颗粒显示出有利的生物安全概况.
生物AgNP是一种生物相容的植物性抗菌剂.

关键词:

抗菌剂抗菌剂抗菌剂鞭毛细胞的运动性离子外流是离子外流.代谢抑制可以抑制代谢.银纳米颗粒银纳米粒子

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