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Updated: Sep 13, 2025

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

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对合成生物学无限制重叠序列克隆技术的DNA审查

Isabella Frighetto Bomfiglio1,2, Isabelli Seiler de Medeiros Mendes1,2, Diego Bonatto1,2

  • 1Laboratório de Biologia Molecular e Computacional, Centro de Biotecnologia da UFRGS, Departamento de Biologia Molecular e Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.

Biotechnology journal
|July 27, 2025
PubMed
概括

本综述探讨了在合成生物学中用于DNA操纵的无限制重叠序列克隆 (RFOSC) 方法. 它评估了各种体外和体内技术,指导研究人员选择最佳的DNA组装策略.

关键词:
在DNA克隆过程中,DNA重叠序列的序列重叠.没有限制的克隆.合成生物学 合成生物学

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