相关实验视频

Updated: Sep 13, 2025

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Development of an Antigen-driven Colitis Model to Study Presentation of Antigens by Antigen Presenting Cells to T Cells

Published on: September 18, 2016

MAIT的细胞对受控口服肠毒性大肠杆菌的反应具有挑战性

Kerri G Lal^1,2, Dohoon Kim^1,2, Matthew Creegan^1,2

¹US Military HIV Research Program, Center for Infectious Diseases Research, Walter Reed Army Institute of Research, Silver Spring, MD, United States.

Journal of immunology (Baltimore, Md. : 1950)

|July 27, 2025

概括

在肠毒性大肠杆菌 (ETEC) 感染期间,粘膜相关的不变T细胞 (MAIT) 激活和扩张. 这种免疫反应,特别是在严重的腹病例中,表明MAIT细胞是ETEC疾病发展的关键参与者.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
胃肠病学胃肠病学

背景情况:

粘膜关联不变T细胞 (MAIT) 对于屏障免疫至关重要,通过MR1分子识别微生物抗原.
肠毒性大肠杆菌 (ETEC) 是全球腹疾病的重要原因,特别是在脆弱人群中.
在ETEC感染中MAIT细胞的作用仍然在很大程度上未被探索.

研究的目的:

在人类挑战模型中研究ETEC感染期间MAIT细胞的动态反应.
为了将MAIT细胞激活,功能和扩张与ETEC感染严重程度相关联.

主要方法:

使用一种受控的人类ETEC挑战模型,使用菌株H10407和B7A.
在挑战后的多个时间点分析了周围血液样本.
在蛋白质和转录水平上评估MAIT细胞表型,功能,增殖和回归潜力 (CCR9,α4β7).

主要成果:

在挑战后的第7天,MAIT细胞的激活,功能和增殖增加,特别是在中度至重度腹 (MSD) 患者中.
在MSD阳性个体中增加的CCR9和α4β7表达表明加强了肠道指导.
在挑战后28天观察到外围MAIT细胞池的显著扩张,与第7天的疾病严重程度相关.

结论:

MAIT细胞对ETEC感染产生全身反应,包括激活和扩张.
MAIT细胞响应的程度与症状ETEC疾病的发展和严重程度有关.
在ETEC感染期间,MAIT细胞被认为是宿主防御和疾病病理学的重要贡献者.

关键词:

欧洲经济共同体欧洲经济共同体一个 MAIT 细胞.在MR1中,MRI是MRI.肠道毒性大肠杆菌 (E. coli) 是一个肠道毒性大肠杆菌.人类人类人类人类人类人类人类

更多相关视频

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Determination of Tolerable Fatty Acids and Cholera Toxin Concentrations Using Human Intestinal Epithelial Cells and BALB/c Mouse Macrophages

Published on: May 30, 2013

相关概念视频

Stringent Response in E. coli

Stringent Response in E. coli

Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...

Chemotaxis in E. coli

Chemotaxis in E. coli

Chemotaxis in Escherichia coli is a sensory-driven motility mechanism that enables bacteria to navigate chemical gradients, moving toward beneficial environments while avoiding harmful conditions. This process relies on a signal transduction system integrating external chemical cues with flagellar motor control.Chemoreceptors and Signal DetectionE. coli detects chemical gradients through methyl-accepting chemotaxis proteins (MCPs), which are membrane-bound chemoreceptors that sense attractants...