柏柏林对M6PR的上调调节通过将STING分类到内分体以降解来减弱肝衰老
Wenjuan Tang1, Qianqian Zhang2, Longyu Qin2
1Department of Gastroenterology, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, PR China; School of Pharmacy, Health Science Center, Xi'an Jiaotong University, Xi'an, Shaanxi, 710061, PR China; State Key Laboratory of Shaanxi for Natural Medicines Research and Engineering, Xi'an, Shaanxi, 710061, PR China.
概括
柏柏林 (BBR) 是一种天然化合物,抑制干扰素基因刺激器 (STING) 信号传递,以减少肝脏衰老. BBR上调曼6-酸盐受体 (M6PR) 以促进STING降解,提供一种新的抗衰老策略.
科学领域:
- 老年学是指老年学的学科.
- 分子生物学分子生物学
- 肝病学 肝病学是一种肝病学.
背景情况:
- 细胞衰老与肝脏疾病有关,干扰素基因刺激器 (STING) 信号驱动衰老和肝星细胞 (HSC) 激活.
- 阻止STING信号传递是一种潜在的抗衰老策略,但针对这种途径的天然化合物尚未得到充分探索.
研究的目的:
- 为了选抑制STING信号传递的天然化合物.
- 研究已识别的化合物的抗衰老作用的药理作用和分子机制.
主要方法:
- 用RT-PCR来识别抑制STING信号的天然化合物.
- 在多克索鲁比诱导的衰老细胞和老化小鼠中评估了抗衰老效应.
- 使用STING通路调节器,二元化试验,生物信息学,西部斑点,免疫沉,免疫光和细胞热转移试验来探索机制.
主要成果:
- 柏柏林 (BBR) 被确定为一种STING抑制剂,具有减弱肝衰老的潜力.
- 在衰老细胞中,BBR上调了曼6酸盐受体 (M6PR),促进了STING结合和内体细胞降解.
- 通过M6PR介导的STING降解对BBR持续的抗衰老作用至关重要,即使在STING表达正常化之后.
结论:
- 柏柏林 (BBR) 是一种有希望的自然化合物,可以缓解肝衰老.
- 曼诺6酸盐受体 (M6PR) 在BBR的抗衰老机制中发挥着至关重要的作用,通过促进内分泌体中的STING降解.
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