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针对人类诱导的多能干细胞定向分化为甲状腺毛囊上皮细胞的协议

Hendrik J Undeutsch¹, Alberto Posabella², Darrell N Kotton³

¹Division of Endocrinology, Diabetes and Metabolism, Joan and Sanford I. Weill Department of Medicine, Weill Cornell Medicine, New York, NY, USA; Department of Medicine, Boston University Chobanian and Avedisian School of Medicine and Boston Medical Center, Boston, MA, USA; Center for Regenerative Medicine, Boston University and Boston Medical Center, Boston, MA, USA.

|July 27, 2025

概括

研究人员开发了一种新的协议,可以从人类干细胞产生甲状腺毛囊细胞 (TFC). 这种方法模仿了自然的甲状腺发育,而不需要额外的转录因子来进行潜在的基于细胞的疗法.

科学领域:

干细胞生物学干细胞生物学
发育生物学是发展生物学.
内分泌学在内分泌学.

背景情况:

甲状腺毛囊细胞 (TFCs) 对于激素的产生至关重要.
在体外生成TFC对于再生医学和疾病建模至关重要.
现有的方法通常需要外源转录因子,使临床应用复杂化.

研究的目的:

从多能干细胞获得人类甲状腺毛囊细胞 (TFCs) 的定向分化协议.
为了回顾甲状腺器官生成的关键发展里程碑 in vitro.
通过各种人类遗传背景验证该协议.

主要方法:

人类诱导的多能干细胞 (iPSC) 或胚胎干细胞被播种和培养.
实现了最终和前肠内皮的诱导.
使用纤维细胞生长因子/骨形态遗传蛋白 (FGF/BMP) 信号进行了甲状腺系谱的规范.
随后的分化和成熟到TFC被监控.

主要成果:

从人类多能干细胞中提取TFC的强有力的协议已建立.
差异化过程成功地回顾了甲状腺形成的发育阶段.
该协议在多个人类干细胞系中显示出有效性,表明其广泛适用性.

结论:

这项研究提出了一种新的,无转录因子的协议,用于从人类iPSCs生成TFC.
该方法有效地模仿胚胎甲状腺发育,为TFC生成提供了一个有希望的途径.
经过验证的协议有可能在甲状腺疾病研究和基于细胞的治疗中得到应用.

关键词:

细胞生物学细胞生物学细胞分化的过程细胞培养培养的细胞培养.显微镜的使用方法分子生物学分子生物学器官类动物器官类动物干细胞是干细胞.

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iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.